PROTEIN GLYCOSYLATION MODIFICATION IN PICHIA PASTORIS

The present invention provides genetically engineered strains of Pichia capable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an agr;-1,2-mannosidase and glucosidase II. The gen...

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Bibliographische Detailangaben
1. Verfasser:	GEYSENS, STEVEN, C., J
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides genetically engineered strains of Pichia capable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an agr;-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoprotiens with reduced glycosylation using such genetically engineered stains of Pichia are also provided. La presente invencion proporciona cepas geneticamente disenadas de Pichia capaces de producir proteinas con glicosilacion reducida. En particular, las cepas geneticamente disenadas de la presente invencion son capaces de expresar cualquiera o ambas de a-1,2- manosidasa y glucosidasa II. Las cepas geneticamente disenadas de la presente invencion pueden ademas modificarse de tal forma que el OCH1 se rompe. Tambien se proporcionan los metodos para producir glicoproteinas con glicosilacion reducida utilizando tales cepas geneticamente disenadas de Pichia.