ELIMINATION OF THE XYLELLA FASTIDIOSA BACTERIA CAUSING THE PIERCE DISEASE BY A CSFV ALONE OR LINKED TO A LYTIC PEPTIDE, AND EXPRESSED IN MUDS OR IN A SOLUBLE FORM

The Xylella fastidiosa bacteria causes the Pierce disease in grape and further crops that are economically important, such as citric, almond, coffee, peach and further crops affected by that bacteria. Anti-Xylella fastidiosa substances were developed including an avian csFv linked to a lytic peptide Cecropin P1 (CP1), which are expressed in a M13 mud, this mud removing 100% the Xylella fastidiosa bacteria in an in-vitro crop, at a concentration of 3.6 x 105 phages/cell after 28 hours of incubation. The invention includes a scFv expressed in a M13 mud, which requires from about 28 hours to about 48 hours to 100% remove the bacteria in an in-vitro crop, and finally a soluble scFv alone or chemically coupled to a lytic peptide CP1 which almost remove in a 100% the Xylella fastidiosa bacteria in an in-vitro crop. The present strategy has a potential application for solving diseases in plants caused by the aforementioned bacteria. Xylella fastidiosa es la causante de la enfermedad de Pierce en la vid y en otros cultivos de importancia economica, como citricos, almendro, cafe, durazno y otros cultivos susceptibles a la bacteria. Se desarrollaron sustancias anti-Xylella fastidiosa compuestas de un csFv de gallina unido a un peptido litico Cecropin P1 (CP1), expresada en un fago M13, que elimina especificamente en un 100% a Xylella fastidiosa en cultivo in vitro, a una concentracion de 3.6 x 105 fagos/celula en 28 horas de incubacion; un scFv expresado en un fago M13, que requiere entre 28 y 48 horas para eliminar a la bacteria en un 100% en cultivo in vitro, y finalmente un scFv soluble solo o acoplado quimicamente al peptido litico CP1 que elimina casi en un 100% a la bacteria Xylella fastidiosa en cultivo in vitro .Esta estrategia tiene una aplicacion potencial para resolver enfermedades en plantas causadas por bacterias.