DNA POLYMERASE OF GAMMA HERPES VIRUSES ASSOCIATED WITH KAPOSI'S SARCOMA AND RETROPERITONEAL FIBROMATOSIS

This invention provides isolated polynucleotides encoding DNA polymerases of three members of a subfamily of gamma herpes viruses. Two were obtained from macaque monkeys affected with retroperitoneal fibromatosis, the other from human AIDS patients affected with Kaposi's sarcoma. A 454-base pai...

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Hauptverfasser:	GEORGE J. TODARO, KURT STRAND, TIMOTHY M. ROSE, MARNIX L. BOSCH
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	This invention provides isolated polynucleotides encoding DNA polymerases of three members of a subfamily of gamma herpes viruses. Two were obtained from macaque monkeys affected with retroperitoneal fibromatosis, the other from human AIDS patients affected with Kaposi's sarcoma. A 454-base pair fragment encoding a region near the active site of the DNA polymerase is 69-83 percent identical amongst the three viruses, but only 54-68 percent identical with other known gamma herpes sequences and < 55 percent identical with alpha and beta herpes sequences. Also provided are ploynucleotides encoding DNA polymerase from related viruses in the RFHV/KSHV subfamily. Polynucleotides prepared according to the sequence data can be used as reagents to detect and characterize related sequences. Such reagents may be used to detect members of the RFHV/KSHV subfamily, including but not limited to RFHV, RFHV2, and KSHV. Corresponding polypeptides and peptide fragments may be obtained by expressing the polynucleotide or by chemical synthesis. They may be used for detecting specific antibody potentially present in the serum of infected subjects. They may also be used for designing or screening pharmaceutical compounds that limit viral replication by inhibiting DNA polymerase activity. Esta invencion proporciona polinucleotidos aislados que codifican ADN polimerasas de tres miembros de una sub-familia de los virus del herpes Gama. Dos fueron obtenidos de monos macacos afectados con fibromatosis retroperitoneal, el otro de pacientes humanos de SIDA afectados con sarcoma de kaposi. Un fragmento de 454 pares de bases codificando una region cercana al sitio activo de la ADN polimerasa es de 69-83% idéntico en los tres virus, pero solamente 54-68% idéntico a otras secuencias de herpes gama conocidas y menos de 55% idéntico a secuencias de herpes alfa y beta. También se proporcionan polinucleotidos que codifican ADN polimerasa de virus relacionados en la sub-familia RFHV/KSHV. Los nucleotidos preparados de acuerdo con los datos de secuencia pueden ser usados como reactivos para detectar y caracterizar secuencias relacionadas. Tales reactivos pueden ser usados para detectar miembros de la sub-familia RFHV/KSHV, incluyendo pero sin limitarse a RFHV, RFHV2 y KSHV. Polipéptidos y fragmentos de péptidos correspondientes pueden ser obtenidos expresando el polinucleotido o mediante síntesis química. Pueden usarse para detectar anticuerpo específico presente en el suero de sujetos infectado