PROCEDURE TO PRODUCE AS WELL AS TO USE ACARVIOSYL-TRANSFERASE IN TRANSFORMATION OF ACARBOSE IN ACARBOSE HOMOLOGUES, FOR ACARBOSE HOMOLOGUES PRODUCTION

The invention refers to Actinomicetes acarviosyl-transferase, mainly from Actinoplanetes species SE 50/110 and their mutants, to a procedure for isolation, purification, and characterization of enzyme, for isolation and characterization of gene acbD which codifies acarviosyl-transferase, to acarvios...

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Hauptverfasser:	HERMANN PAPE, MICHAEL HEMKER, ANNELIESE CRUEGER, HANS-GEORG DELLWEG, JURGEN GEORG LENZ, KLAUS GOEKE, WOLFGANG PIEPERSBERG, WERNER SCHRODER, BEATE SCHAPER
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention refers to Actinomicetes acarviosyl-transferase, mainly from Actinoplanetes species SE 50/110 and their mutants, to a procedure for isolation, purification, and characterization of enzyme, for isolation and characterization of gene acbD which codifies acarviosyl-transferase, to acarviosyl-transferase expression in a heterologous host, to use of acarviosyl-transferase for transformation of acarbose in acarbose derived components, or else for acarbose homologues production, to acarviosyl-transferase use in acarbose purification, as well as productive mutants obtainment with derived components formation reduced by acarviosyl-transferase gene inactivation. La invencion se refiere a la acarviosil-transferasa de Actinomicetes, pricipalmente de especies Actinoplanetes SE 50/110 y sus mutantes, a un procedimiento para el aislamiento, purificacion y caracterizacion de la enzima, para el aislamiento y caracterizacion del gen acbD que codifica la acarviosil-transferasa, a la expresion de la acarviosil-transferasa en un organismo hospedador heterologo, al uso de la acarviosil-transferasa para la transformacion de componentes derivados de acarbosa en acarbosa o bien para la produccion de homologos de la acarbosa, al uso de la acarviosil-transferasa en la purificacion de acarbosa, así como la obtencion de mutantes productivos con formacion de componentes derivados reducida mediante inactivacion del gen de la acarviosil-transferasa.