AGLYCOSYLATED Fc-CONTAINING POLYPEPTIDES

Provided herein are variant human lgG1 Fe molecules lacking or with highly reduced effector function and high stability despite lacking glycosylation at N297. Also, provided herein are linker peptides that are glycosylated when expressed in mammalian cells. IL-2 binds three transmembrane receptor subunits: IL-2R- and IL-2Rywhich together activate intracellular signaling events upon IL-2 binding, and CD25 (IL-2Ra) which serves to stabilize the interaction between IL-2 and IL-2RBY.The signals delivered by IL-2RBYinclude those of the PI3-kinase, Ras-MAP-kinase, and STAT5 pathways. La presente invención se refiere a moléculas IgG1 Fc humanas variantes que carecen o con función efectora sumamente reducida y alta estabilidad a pesar de que carece de glicosilación en N297. También, se proporcionan en la presente péptidos ligadores que se glicosilan cuando se expresan en las células de mamífero.