A WHOLE, LEECH SALIVA EXTRACT

Methods are provided for isolating and using a whole-saliva leech extract. The methods can include feeding a phagostimulatory agent to a leech; inducing a regurgitation in the leech, the inducing including placing the leech in an environment having a temperature of less than about 0°C; and, collecting an unrefined, whole saliva in the regurgitation of the cooled leech. The methods can include revitalizing the leech by warming it at a temperature ranging from about 5°C to about 40°C. Stable, lyophilized, whole-saliva extracts of a leech are also provided, the extract having a stable activity when stored for use at a temperature below about -20°C, the extract maintaining at least 70% of the activity for at least 6 months. The extracts can be used to treat solid tumors, treat liquid tumors, treat diabetes, treat a viral disease, treat a parasitic disease, treat an antibacterial disease, or serve as an anti-oxidant. La presente invención se refiere a el uso de una cantidad eficaz de un extracto de saliva entera de una sanguijuela en la fabricación de un medicamento para el tratamiento de hipoglucemia diabética, en donde el extracto se obtiene al: alimentar con un agente fagoestimulador a la sanguijuela; inducir una regurgitación en la sanguijuela en un medio que tiene una temperatura de menos de 0°C; recolectar una saliva entera no refinada en la regurgitación de la sanguijuela fría; separar componentes sólidos de la saliva entera no refinada para crear, una saliva entera refinada; y, liofilizar el extracto de saliva entera.