POLYPEPTIDES WITH LIPASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME

The present invention relates to isolated polypeptides with lipase activity, selected from the group consisting of:(a)a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at leas...

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Hauptverfasser: Lone BAUNSGAARD, Carsten Hoerslev HANSEN, Vikram Kjoeller BHATIA, Marc Dominique MORANT, Anna Verena REISER, Kim BORCH
Format: Patent
Sprache:eng ; spa
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Zusammenfassung:The present invention relates to isolated polypeptides with lipase activity, selected from the group consisting of:(a)a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 4;(b)a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) SEQ ID NO: 3or the full-length complement of (i);(c)a polypeptide encoded by a polynucleotide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 3;(d)a polypeptide which is a variant of SEQ ID NO: 4 comprising a substitution, deletion, and/or insertion at one or more (e.g. several) positions; and(e)a polypeptide which is a fragment of any of the polypeptides of (a), (b), (c) or (d).The invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides. La presente invención se refiere a polipéptidos aislados con actividad lipasa, seleccionados del grupo que consiste en: (a) un polipéptido que tiene una identidad de secuencia de al menos un 75%, al menos un 80%, al menos un 85%, al menos un 90%, al menos un 91%, al menos un 92%, al menos un 93%, al menos un 94%, al menos un 95%, al menos un 96%, al menos un 97%, al menos un 98%, al menos un 99%, o un 100% respecto de la SEQ ID NO: 4; (b) un polipéptido codificado por un polinucleótido que se hibrida en condiciones de rigurosidad baja, condiciones de rigurosidad media, condiciones de rigurosidad media-alta, condiciones de rigurosidad alta, o condiciones de rigurosidad muy alta con (i) la SEQ ID NO: 3 o el complemento de longitud completa de (i); (c) un polipéptido codificado por un polinucleótido que tiene una identidad de secuencia de al menos un 75%, al menos un 80%, al menos un 85%, al menos un 90%, al menos un 91%, al menos un 92%, al menos un 93%, al menos un 94%, al menos un 95%, al menos un 96%, al menos un 97%, al menos un 98%, al menos un 99%, o un 100% respecto de la SEQ ID NO: 3; (d) un polipéptido que es una variante de