ALPHA-AMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME

The present invention relates to variants of a parent alpha-amylase. The present invention also relates to polynucleotides encoding the variants and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variant enzymes. La presente invención se...

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Bibliographische Detailangaben
Hauptverfasser:	Carsten ANDERSEN, Randall DEINHAMMER, Anders VIKSOE-NIELSEN, Lars Lehmann Hylling CHRISTENSEN, Thomas Agersten POULSEN, Peter Kamp HANSEN, Signe E. LARSEN, Miguel DUARTE TOSCANO, Henrik FRIIS-MADSEN
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY CANDLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DETERGENT COMPOSITIONS DETERGENTS ENZYMOLOGY FATTY ACIDS THEREFROM FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECOVERY OF GLYCEROL RESIN SOAPS SOAP OR SOAP-MAKING SPIRITS USE OF SINGLE SUBSTANCES AS DETERGENTS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to variants of a parent alpha-amylase. The present invention also relates to polynucleotides encoding the variants and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the variant enzymes. La presente invención se refiere a una variante de alfa-amilasa, caracterizada porque comprende el conjunto de sustituciones en las posiciones E129V+K177L+R179E y por lo menos una sustitución de aminoácidos adicional en una posición que corresponde a cualquiera de las posiciones 59, 89, 91, 96, 108, 112, 157, 165, 166, 171, 180, 181, 184, 208, 220, 224, 242, 254, 269, 270, 274, 276, 281, 284, 416, y 427, en donde la variante tiene por lo menos 90% y menos de 100% de identidad de secuencia con el polipéptido maduro de la SEQ ID. NO.1, en donde la variante tiene actividad de alfa-amilasa.