PRODUCTION OF OMEGA AMINO FATTY ACIDS

Whole cell catalyst expressing a recombinant alpha -dioxygenase or a combination of a recombinant fatty acid reductase and a recombinant phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and recombinant transaminase, is claimed. Independent claims are also included f...

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Hauptverfasser:	Dr. Steffen SCHAFFER, Dr. Harald HÄGER, Dr. Thomas HAAS, Dr. Wilfried BLÜMKE, Dr. Mirja WESSEL, Jasmin GIELEN, Dr. Hans-Georg HENNEMANN
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE VINEGAR WINE
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Beschreibung
Zusammenfassung:	Whole cell catalyst expressing a recombinant alpha -dioxygenase or a combination of a recombinant fatty acid reductase and a recombinant phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and recombinant transaminase, is claimed. Independent claims are also included for: (1) reacting a fatty acid, omega -hydroxy fatty acid, omega -oxo fatty acid or their monoester to an amine, comprising (a) oxidizing the fatty acid, omega -hydroxy fatty acid, omega -oxo fatty acid or their monoester by contacting them with an alkane hydroxylase and/or an alcohol dehydrogenase to obtain an oxidation product, (b) contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or an alpha -dioxygenase to obtain an aldehyde, and (c) contacting the aldehyde with a transaminase; and (2) a reaction mixture comprising the above whole cell catalyst in an aqueous solution and the fatty acid, omega -hydroxy fatty acid, omega -oxo fatty acid or their monoester. La presente invención se refiere a un catalizador de célula completa que expresa una a-dioxigenasa recombinante o la combinación de una reductasa recombinante de ácido graso y una fosfopanteteinil transferasa que fosfopanteteiniliza la reductasa de ácido graso, y que además de la a-dioxigenasa y/o la combinación de la reductasa de ácido graso y la fosfopanteteinil transferasa expresa una transaminasa, caracterizado en que la fosfopanteteinil transferasa y/o la transaminasa son preferentemente recombinantes; y a un método para la conversión de un ácido graso, un ácido graso ?-hidroxi, un ácido graso ?-oxo o un monoéster de los mismos en una amina, que comprende las etapas de oxidación del ácido graso, el ácido graso ?-hidroxi, el ácido graso ?-oxo o el monoéster de los mismos a un producto de oxidación al ponerlo en contacto con un alcano hidroxilasa y/o un alcohol deshidrogenasa, poniendo en contacto el producto de la oxidación con una reductasa de ácido graso fosfopanteteinilizada o una a-dioxigenasa para proporcionar un aldehído, y poner en contacto el aldehído con una transaminasa.