NOVEL XYLOSE TRANPORTERS AND THEIR APPLICATIONS THEREOF

Described is a novel transporter for five-carbon sugar best known as xylose, which corresponds to a protein obtained from the Escherichia coli bacterium, also referring to the DNA encoding for said protein (gene gatC, SEQ. ID NO: 13) and mutant protein with a better transport capacity (gen gatCS184L...

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Hauptverfasser:	JOSÉ UTRILLA CARRERI, LUZ MARÍA MARTÍNEZ MEJÍA, GUILLERMO GOSSET LAGARDA, ALFREDO MARTÍNEZ JIMÉNEZ
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Described is a novel transporter for five-carbon sugar best known as xylose, which corresponds to a protein obtained from the Escherichia coli bacterium, also referring to the DNA encoding for said protein (gene gatC, SEQ. ID NO: 13) and mutant protein with a better transport capacity (gen gatCS184L, SEQ. ID NO: 14). The present invention shows the capacity of the protein GatC (SEQ. ID NO: 15) for transporting xylose. Present is a mutant protein which transforms a Serine into a Leucine, a polar amino acid into a non polar amino acid, in the amino acid 184 (GatCS184L, SEQ. ID NO: 16) the improved capacity thereof to transport xylose is shown. As a consequence of the need of xylose transporting proteins that are energetically favourable, the present invention suggests the use of the product resulting from the genes gatC (SEQ. ID NO: 13) and gatCS184L (SEQ. ID NO: 14) as transporters in organisms searching the property of xylose transport or the improvement thereof for the utilization of said organism in the conversion of xylose-rich syrups, such as those obtained in the lignocelluloses hydrolysis as a raw material. La presente invención se refiere a un novedoso transportador del azúcar de cinco carbonos denominado xilosa, que corresponde a una proteína obtenida de la bacteria Escherichia coli; así mismo, se refiere al ADN codificante de dicha proteína (gen gatC, SEQ. ID NO: 13) y una mutante con mejor capacidad de transporte (gen gatCS184L, SEQ. ID NO: 14). En la presente invención se demuestra la capacidad de la proteína GatC (SEQ. ID NO: 15) para transportar xilosa. Se presenta una mutante que provoca el cambio de una Serina por una Leucina, aminoácido polar por uno no polar, en el aminoácido 184 (GatCS184L, SEQ. ID NO: 16) y se demuestra su capacidad mejorada de transporte de xilosa. Debido a que existe una necesidad de proteínas transportadoras de xilosa que sean energéticamente favorables, en la presente invención se plantea el uso del producto de los genes gatC (SEQ. ID NO: 13) y gatCS184L (SEQ. ID NO: 14) como transportadores en organismos donde se busque otorgar la propiedad de transporte de xilosa o bien su mejoramiento para la utilización de dichos organismos en la conversión de jarabes ricos en xilosa, tales como los obtenidos de la hidrólisis de lignocelulosa como materia prima. Figura 3.