STABLE PROTEASE VARIANTS

The present invention relates to a protease variant which is at least 90% identical to the full length amino acid sequence of a Kumamolisin AS backbone as set forth in any of SEQ ID NOs 1 - 3, while maintaining proteolytic activity, or a fragment, fraction or shuffled variant thereof maintaining pro...

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Bibliographische Detailangaben
Hauptverfasser:	SCHEIDIG Andreas, KRAPP Claudia, HORN Thomas, MICHELS Andreas, ELEND Christian
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY CANDLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DETERGENT COMPOSITIONS DETERGENTS ENZYMOLOGY FATTY ACIDS THEREFROM FODDER FOODS OR FOODSTUFFS HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS RECOVERY OF GLYCEROL RESIN SOAPS SOAP OR SOAP-MAKING SPIRITS THEIR TREATMENT, NOT COVERED BY OTHER CLASSES USE OF SINGLE SUBSTANCES AS DETERGENTS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a protease variant which is at least 90% identical to the full length amino acid sequence of a Kumamolisin AS backbone as set forth in any of SEQ ID NOs 1 - 3, while maintaining proteolytic activity, or a fragment, fraction or shuffled variant thereof maintaining proteolytic activity, which protease variant demonstrates altered or improved stability compared to the Kumamolisin AS wildtype as set forth in SEQ ID NO 4, or the Kumamolisin AS backbone as set forth in any of SEQ ID NOs 1 - 3. La presente invención se refiere a una variante de proteasa que es al menos 90% idéntica a la secuencia de aminoácidos de longitud completa de una estructura de la base Kumamolisina AS como se establece en cualquiera de las Id. de Sec. nos. de 1 - 3, mientras que mantiene la actividad proteolítica, o un fragmento, fracción o variante combinada de la misma que mantiene la actividad proteolítica, el cual variante de proteasa demuestra estabilidad alterada o mejorada en comparación con la Kumamolisina AS de tipo silvestre como se expone en Id. de Sec. no. 4, o la estructura de la base Kumamolisina AS como se establece en cualquiera de las Id. de Sec. nos. de 1 - 3.