COMPOSITIONS AND METHODS FOR PRODUCING REBAUDIOSIDE D

The present disclosure provides enzymes and a method to use those enzymes to transfer a sugar moiety to a substrate steviol glycoside. Specifically, designed beta-1,2-glycosyltransferases and sucrose synthases are used in a one-pot reaction to convert stevioside and Reb A into Reb E and Reb D. La pr...

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Bibliographische Detailangaben
Hauptverfasser:	BAN Yih- En Andrew, ZANGHELLINI Alexandre, KRISTIANSEN Niklas Dalgas, NANCE Mark, GRABS Daniela, ROBERTS Kyle Eugene, HAVRANEK James J, DEVKOTA Ashwini
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present disclosure provides enzymes and a method to use those enzymes to transfer a sugar moiety to a substrate steviol glycoside. Specifically, designed beta-1,2-glycosyltransferases and sucrose synthases are used in a one-pot reaction to convert stevioside and Reb A into Reb E and Reb D. La presente descripción proporciona enzimas y un método para usar esas enzimas para transferir un resto de azúcar a un glucósido de esteviol de sustrato. Específicamente, las beta-1,2-glucosiltransf erasas y sacarosa sintasas diseñadas se utilizan en una reacción de un solo recipiente para convertir el esteviósido y Reb A en Reb E y Reb D.