METHODS FOR TRANSDUCING IMMUNE CELLS

Provided herein are improved methods for transducing immune cells, such as T cells, with retroviral vectors to express exogenous gene products, such as chimeric antigen receptors (CARs). Provided herein are methods that increase transduction efficiency thereby increasing the percentage of immune cel...

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Bibliographische Detailangaben
Hauptverfasser:	ZHANG Chupei, NI Yajin, GERMANSDERFER Abraham, RAO Suma, LUMAN Todd, ZARAGOZA Josergio, HUANG Tom Tao
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided herein are improved methods for transducing immune cells, such as T cells, with retroviral vectors to express exogenous gene products, such as chimeric antigen receptors (CARs). Provided herein are methods that increase transduction efficiency thereby increasing the percentage of immune cells in a population expressing the exogenous gene product. Associated cells, cell populations, compositions and methods of use are also provided. En la presente descripción se proporcionan métodos mejorados para transducir células inmunitarias, tales como células T, con vectores retrovíricos para expresar productos génicos exógenos, tales como receptores de antígenos quiméricos (CAR). En la presente descripción se proporcionan métodos que aumentan la eficiencia de transducción, lo que aumenta de esta manera el porcentaje de células inmunitarias en una población que expresa el producto génico exógeno. También se proporcionan células asociadas, poblaciones celulares, composiciones y métodos de uso.