P450 BM3 MONOOXYGENASE VARIANTS FOR C19-HYDROXYLATION OF STEROIDS

The present invention relates to novel recombinant Bacillus megaterium cytochrome P450-monooxygenase (P450-BM3) variants for the C19 hydroxylation of steroids and derivatives thereof or for improved BM3 protein expression. In particular, the present invention also relates to methods and processes us...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	KENSCH Oliver, RICHTER Florian, SKALDEN Lilly, HELFRICH Petra, BULUT Dalia, PILLING Jens, LINNHOFF Ruben, WAGNER Jakob, KRETSCHMANN Nils, ZORN Ludwig, THEDE Kai, LUDWIG Marcus, BERENDES Frank, COCO Wayne, TRENNER Sabine, BURMEISTER Jens
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The present invention relates to novel recombinant Bacillus megaterium cytochrome P450-monooxygenase (P450-BM3) variants for the C19 hydroxylation of steroids and derivatives thereof or for improved BM3 protein expression. In particular, the present invention also relates to methods and processes using P450-BM3 variants for the production of estrone and estradiol. The invention further relates to nucleotide sequences, constructs and vectors for the expression of these P450-BM3 variants. La presente invención se refiere a nuevas variantes recombinantes de citocromo P450-monooxigenasa (P450-BM3) de Bacillus megaterium para la hidroxilación C19 de esteroides y derivados de los mismos, o para mejorar la expresión de la proteína BM3. En particular, la presente invención también se refiere a métodos y procesos que usan variantes de P450-BM3 para la producción de estrona y estradiol. La invención se relaciona además con secuencias de nucleótidos, construcciones y vectores para la expresión de estas variantes de P450-BM3.