AMORPHA-4,11-DIENE 12-MONOOXYGENASE VARIANTS AND USES THEREOF

AMORPHA-4,11-DIENE 12-MONOOXYGENASE VARIANTS AND USES THEREOF

Provided herein are genetically modified host cells, compositions, and methods for improved production of artemisinic acid. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of amorpha-4,11-diene 12-monooxygenase. Also provided...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: JIANG Hanxiao, PADDON Christopher J, KUNG Stephanie H
Format: Patent
Sprache:eng ; spa
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Provided herein are genetically modified host cells, compositions, and methods for improved production of artemisinic acid. The host cells are genetically modified to contain a heterologous nucleic acid that expresses novel and optimized variants of amorpha-4,11-diene 12-monooxygenase. Also provided herein are methods for screening for variants of cytochrome p450 enzymes that have increased enzymatic activity relative to a parental control enzyme. En el presente documento se proporcionan composiciones, métodos y células hospedadoras modificadas genéticamente para mejorar la producción de ácido artemisínico. Las células hospedadoras se modifican genéticamente para que contengan un ácido nucleico heterólogo que expresa variantes nuevas y optimizadas de amorfa-4,11-dieno 12-monooxigenasa. También se proporcionan en este documento métodos para cribar variantes de las enzimas del citocromo p450 que tienen una actividad enzimática aumentada en relación con una enzima de control original.