FED-BATCH IN VITRO TRANSCRIPTION PROCESS

The present disclosure provides methods of in vitro transcribing a ribonucleic acid (RNA) of interest. In some embodiments, such methods include determining consumption rates of nucleoside triphosphates (NTPs). La presente descripción proporciona métodos de transcripción in vitro de un ácido ribonuc...

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Bibliographische Detailangaben
Hauptverfasser: ELICH Joseph, RABIDEAU Amy E, WOJCIECHOWSKI Peter, PHILPOT Rosalie, FRITZ Brian, SHAMASHKIN Michael
Format: Patent
Sprache:eng ; spa
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Zusammenfassung:The present disclosure provides methods of in vitro transcribing a ribonucleic acid (RNA) of interest. In some embodiments, such methods include determining consumption rates of nucleoside triphosphates (NTPs). La presente descripción proporciona métodos de transcripción in vitro de un ácido ribonucleico (ARN) de interés; en algunas modalidades, dichos métodos incluyen determinar velocidades de consumo de trifosfatos de nucleósidos (NTP).