HIGH-PURITY PRODUCTION OF MULTI-SUBUNIT PROTEINS SUCH AS ANTIBODIES IN TRANSFORMED MICROBES SUCH AS PICHIA PASTORIS

Methods for producing heterologous multi-subunit proteins in transformed cells are disclosed. In particular, the present disclosure provides improved methods of producing multi-subunit proteins, including antibodies and other multi-subunit proteins, which may or may not be secreted, with a higher yi...

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Bibliographische Detailangaben
Hauptverfasser:	Leon F. GARCIA MARTINEZ, Patricia Dianne MCNEILL, Pei QI, Nicole JANSON, John A. LATHAM, Gary L. LESNICKI
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Methods for producing heterologous multi-subunit proteins in transformed cells are disclosed. In particular, the present disclosure provides improved methods of producing multi-subunit proteins, including antibodies and other multi-subunit proteins, which may or may not be secreted, with a higher yield and decreased production of undesired side-products. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris. Se describen métodos para producir proteínas de múltiples subunidades heterólogas en células transformadas. En particular, la presente descripción proporciona métodos mejorados para producir proteínas de múltiples subunidades, incluyendo anticuerpos y otras proteínas de múltiples subunidades, que pueden o no ser secretadas, con mayor rendimiento y menor producción de subproductos indeseados. En ejemplos de modalidades, las células transformadas son una levadura, por ej., levadura metilotrófica tal como Pichia pastoris.