PROCESS FOR PRODUCING A RHAMNOLIPID PRODUCED BY PSEUDOMONAS OR ENTEROBACTER USING ANDIROBA OR MURUMURU SEED WASTE
Process for producing a rhamnolipid produced by Pseudomonas or Enterobacter using andiroba or murumuru seed waste, pertaining to the sector of compounds containing monosaccharide radicals, consists of producing rhamnolipids by a biotechnological process using andiroba or murumuru seed waste, followi...
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Zusammenfassung: | Process for producing a rhamnolipid produced by Pseudomonas or Enterobacter using andiroba or murumuru seed waste, pertaining to the sector of compounds containing monosaccharide radicals, consists of producing rhamnolipids by a biotechnological process using andiroba or murumuru seed waste, following oil extraction, as a substrate for a Pseudomonas aeruginosa, Enterobacter hormaechei or Enterobacter buriae line cultivated in a bioreactor with a non-dispersive aeration system for reducing foam, producing a rhamnolipid content of 10.5 g/L for Pseudomonas aeruginosa bacteria, in bioreactors carried out in a stirred tank with non-dispersive aeration using microporous membranes, particularly of silicone tubes, which allow oxygen to be supplied by diffusion. This type of aeration allows for various configurations, and in the embodiment of the invention, the porous membrane/tube was internally located in the liquid in the bioreactor in the form of a serpentine, under the following process conditions: pure oxygen with suitable pressure and flow rate to maintain O2 pressure in the bioreactor at 20% during the first 24 hours of the assay and stirring varying from 300 to 700 rpm, using 2 radial impellers and manual adjustment according to the decrease in the concentration of dissolved oxygen. The product produced has features that can be used primarily in the cosmetic industry due to its emulsifying, stability and non toxicity capacities.
Proceso de obtención de un ramnolípido producido por pseudomonas o enterobacter usando el residuo de semilla de andiroba o murumuru perteneciente al sector de compuestos que contienen radicales monosacáridos, que consiste en la obtención de ramnolípidos por medio de un proceso biotecnológico usando el residuo de semilla de andiroba o murumuru, después de la extracción do su aceite, como sustrato para el linaje de Pseudomonas aeruginosa o Enterobacter hormaechei o Enterobacter buriae cultivado en un biorreactor con un sistema de aireación no dispersiva para la reducción de la espuma, obteniéndose una cantidad de ramnolípido de 10,5 g/L para las bacterias Pseudomonas aeruginosa, en biorreactores llevados a cabo en un tanque agitado con aireación no dispersiva usándose membranas microporosas, particularmente de tubos de silicona, que permiten el suministro de oxígeno por difusión. Este tipo de aireación permite diferentes configuraciones, siendo que en el ejemplo de modalidad de la invención, la membrana / tubo poroso fue localizada i |
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