CLONING, EXPRESSION AND USE OF ACID PHOSPHOLIPASES
The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID No: 1, b) DNA sequences that comp...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | eng ; spa |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | TATIANA SCHWARZ QUOC KHANH NGUYEN KORNELIA TITZE VOLKER MARSCHNER SILVIA PALADINO PATRICK LORENZ |
| description | The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID No: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for with the restriction map by the pPL3940-Topo2.5 plasmid according to figure 7 and that are stored under number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) in the basis of the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: a or a sequence derived therefrom, which can be obtained by substituting, adding, or deleting one or more amino acids, c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide for which a nucleic acid sequence codes, which is hybridized under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acids, f) allelic variants for amino acid sequences a) to e).
La invención se refiere a una secuencia de ADN que codifica para un polipéptido que tiene actividad de fosfolipasa, esencialmente sin actividad de lipasa, caracterizado porque la secuencia de ADN es seleccionada de a) secuencias de ADN que contienen una secuencia de nucleótidos de conformidad con SEQ ID NO: 1, b) secuencias de ADN que contienen la secuencia codificadora de conformidad cori SEQ ID NO: 1, c) secuencias de ADN que c |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_MX2012004725A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>MX2012004725A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_MX2012004725A3</originalsourceid><addsrcrecordid>eNrjZDBy9vH38_Rz11FwjQgIcg0O9vT3U3D0c1EIDXZV8HdTcHT2dFEI8PAPBmIfzwDHYNdgHgbWtMSc4lReKM3NoOTmGuLsoZtakB-fWlyQmJyal1oS7xthZGBoZGBgYm5k6mhMlCIAuI4nLw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>CLONING, EXPRESSION AND USE OF ACID PHOSPHOLIPASES</title><source>esp@cenet</source><creator>TATIANA SCHWARZ ; QUOC KHANH NGUYEN ; KORNELIA TITZE ; VOLKER MARSCHNER ; SILVIA PALADINO ; PATRICK LORENZ</creator><creatorcontrib>TATIANA SCHWARZ ; QUOC KHANH NGUYEN ; KORNELIA TITZE ; VOLKER MARSCHNER ; SILVIA PALADINO ; PATRICK LORENZ</creatorcontrib><description>The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID No: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for with the restriction map by the pPL3940-Topo2.5 plasmid according to figure 7 and that are stored under number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) in the basis of the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: a or a sequence derived therefrom, which can be obtained by substituting, adding, or deleting one or more amino acids, c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide for which a nucleic acid sequence codes, which is hybridized under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acids, f) allelic variants for amino acid sequences a) to e).
La invención se refiere a una secuencia de ADN que codifica para un polipéptido que tiene actividad de fosfolipasa, esencialmente sin actividad de lipasa, caracterizado porque la secuencia de ADN es seleccionada de a) secuencias de ADN que contienen una secuencia de nucleótidos de conformidad con SEQ ID NO: 1, b) secuencias de ADN que contienen la secuencia codificadora de conformidad cori SEQ ID NO: 1, c) secuencias de ADN que codifican la secuencia de la proteína de conformidad con SEQ ID NO: 2, d) secuencias de ADN que son codificadas por el plásmido pPL3940-Topo2.5 con el mapa de restricción de conformidad con la Figura 7, el cual está depositado bajo el número de acceso DSM 22741, e) secuencias de ADN que hibridan bajo condiciones astringentes con una de las secuencias de ADN de conformidad con a), b), c) o d), f) secuencias de ADN que están relacionadas con las secuencias de ADN de conformidad con a), b), c), d) o e) debido a la degeneración del código genético, y g) cadenas complementarias a las secuencias de conformidad con a) a f), caracterizadas porque la secuencia de ADN preferentemente es derivada de Aspergillus y más preferiblemente de Aspergillus fumigalzus, y un polipéptido que tiene actividad de fosfolipasa, esencialmente sin actividad de lipasa seleccionado de a) un polipéptido que es codificado por la parte codificadora de una secuencia de ADN como se ha definido anteriormente, b) un polipéptido que tiene la secuencia de conformidad con SEQ ID NO: 2 o una secuencia derivada de ésta, la cual puede ser obtenida por substitución adición, deleción de uno o más aminoácidos, c) un polipéptido que tiene una secuencia que tiene al menos 83% de identidad con los aminoácidos 1 a 299 de SEQ ID NO: 2, d) un polipéptido que es codificado por una secuencia de ácido nucleico que hibrida bajo condiciones astringentes con (i) los nucleótidos 55 a 1106 de SEQ ID NO: 1, (ii) la secuencia de ADNC contenida en los nucleótidos 55 a 1106 de SEQ ID NO: 1, (iii) una secuencia parcial de (1) o (ii) compuesta por al menos 100 nucleótidos, o (iv) una cadena complementaria de (i), (ii) o (iii), e) una variante del polipéptido que tiene SEQ ID NO: 2 conteniendo una substitución, deleción y/o inserción de uno o más aminoácidos, f) variantes alélicas de las secuencias de aminoácidos a) a e).</description><language>eng ; spa</language><subject>ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES ; BEER ; BIOCHEMISTRY ; BLEACHING LEATHER OR FURS ; CANDLES ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; DETERGENTS ; DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS,YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS ; ENZYMOLOGY ; ESSENTIAL OILS ; FATTY ACIDS THEREFROM ; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR ; LAUNDERING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PERFUMES ; PRODUCING (PRESSING, EXTRACTION), REFINING AND PRESERVINGFATS, FATTY SUBSTANCES (e.g. LANOLIN), FATTY OILS AND WAXES,INCLUDING EXTRACTION FROM WASTE MATERIALS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TEXTILES ; TREATMENT OF TEXTILES OR THE LIKE ; VINEGAR ; WINE</subject><creationdate>2012</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120529&DB=EPODOC&CC=MX&NR=2012004725A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120529&DB=EPODOC&CC=MX&NR=2012004725A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TATIANA SCHWARZ</creatorcontrib><creatorcontrib>QUOC KHANH NGUYEN</creatorcontrib><creatorcontrib>KORNELIA TITZE</creatorcontrib><creatorcontrib>VOLKER MARSCHNER</creatorcontrib><creatorcontrib>SILVIA PALADINO</creatorcontrib><creatorcontrib>PATRICK LORENZ</creatorcontrib><title>CLONING, EXPRESSION AND USE OF ACID PHOSPHOLIPASES</title><description>The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID No: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for with the restriction map by the pPL3940-Topo2.5 plasmid according to figure 7 and that are stored under number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) in the basis of the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: a or a sequence derived therefrom, which can be obtained by substituting, adding, or deleting one or more amino acids, c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide for which a nucleic acid sequence codes, which is hybridized under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acids, f) allelic variants for amino acid sequences a) to e).
La invención se refiere a una secuencia de ADN que codifica para un polipéptido que tiene actividad de fosfolipasa, esencialmente sin actividad de lipasa, caracterizado porque la secuencia de ADN es seleccionada de a) secuencias de ADN que contienen una secuencia de nucleótidos de conformidad con SEQ ID NO: 1, b) secuencias de ADN que contienen la secuencia codificadora de conformidad cori SEQ ID NO: 1, c) secuencias de ADN que codifican la secuencia de la proteína de conformidad con SEQ ID NO: 2, d) secuencias de ADN que son codificadas por el plásmido pPL3940-Topo2.5 con el mapa de restricción de conformidad con la Figura 7, el cual está depositado bajo el número de acceso DSM 22741, e) secuencias de ADN que hibridan bajo condiciones astringentes con una de las secuencias de ADN de conformidad con a), b), c) o d), f) secuencias de ADN que están relacionadas con las secuencias de ADN de conformidad con a), b), c), d) o e) debido a la degeneración del código genético, y g) cadenas complementarias a las secuencias de conformidad con a) a f), caracterizadas porque la secuencia de ADN preferentemente es derivada de Aspergillus y más preferiblemente de Aspergillus fumigalzus, y un polipéptido que tiene actividad de fosfolipasa, esencialmente sin actividad de lipasa seleccionado de a) un polipéptido que es codificado por la parte codificadora de una secuencia de ADN como se ha definido anteriormente, b) un polipéptido que tiene la secuencia de conformidad con SEQ ID NO: 2 o una secuencia derivada de ésta, la cual puede ser obtenida por substitución adición, deleción de uno o más aminoácidos, c) un polipéptido que tiene una secuencia que tiene al menos 83% de identidad con los aminoácidos 1 a 299 de SEQ ID NO: 2, d) un polipéptido que es codificado por una secuencia de ácido nucleico que hibrida bajo condiciones astringentes con (i) los nucleótidos 55 a 1106 de SEQ ID NO: 1, (ii) la secuencia de ADNC contenida en los nucleótidos 55 a 1106 de SEQ ID NO: 1, (iii) una secuencia parcial de (1) o (ii) compuesta por al menos 100 nucleótidos, o (iv) una cadena complementaria de (i), (ii) o (iii), e) una variante del polipéptido que tiene SEQ ID NO: 2 conteniendo una substitución, deleción y/o inserción de uno o más aminoácidos, f) variantes alélicas de las secuencias de aminoácidos a) a e).</description><subject>ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>BLEACHING LEATHER OR FURS</subject><subject>CANDLES</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>DETERGENTS</subject><subject>DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS,YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS</subject><subject>ENZYMOLOGY</subject><subject>ESSENTIAL OILS</subject><subject>FATTY ACIDS THEREFROM</subject><subject>FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR</subject><subject>LAUNDERING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PERFUMES</subject><subject>PRODUCING (PRESSING, EXTRACTION), REFINING AND PRESERVINGFATS, FATTY SUBSTANCES (e.g. LANOLIN), FATTY OILS AND WAXES,INCLUDING EXTRACTION FROM WASTE MATERIALS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TEXTILES</subject><subject>TREATMENT OF TEXTILES OR THE LIKE</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2012</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDBy9vH38_Rz11FwjQgIcg0O9vT3U3D0c1EIDXZV8HdTcHT2dFEI8PAPBmIfzwDHYNdgHgbWtMSc4lReKM3NoOTmGuLsoZtakB-fWlyQmJyal1oS7xthZGBoZGBgYm5k6mhMlCIAuI4nLw</recordid><startdate>20120529</startdate><enddate>20120529</enddate><creator>TATIANA SCHWARZ</creator><creator>QUOC KHANH NGUYEN</creator><creator>KORNELIA TITZE</creator><creator>VOLKER MARSCHNER</creator><creator>SILVIA PALADINO</creator><creator>PATRICK LORENZ</creator><scope>EVB</scope></search><sort><creationdate>20120529</creationdate><title>CLONING, EXPRESSION AND USE OF ACID PHOSPHOLIPASES</title><author>TATIANA SCHWARZ ; QUOC KHANH NGUYEN ; KORNELIA TITZE ; VOLKER MARSCHNER ; SILVIA PALADINO ; PATRICK LORENZ</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_MX2012004725A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; spa</language><creationdate>2012</creationdate><topic>ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>BLEACHING LEATHER OR FURS</topic><topic>CANDLES</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>DETERGENTS</topic><topic>DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS,YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS</topic><topic>ENZYMOLOGY</topic><topic>ESSENTIAL OILS</topic><topic>FATTY ACIDS THEREFROM</topic><topic>FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR</topic><topic>LAUNDERING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PERFUMES</topic><topic>PRODUCING (PRESSING, EXTRACTION), REFINING AND PRESERVINGFATS, FATTY SUBSTANCES (e.g. LANOLIN), FATTY OILS AND WAXES,INCLUDING EXTRACTION FROM WASTE MATERIALS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TEXTILES</topic><topic>TREATMENT OF TEXTILES OR THE LIKE</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>TATIANA SCHWARZ</creatorcontrib><creatorcontrib>QUOC KHANH NGUYEN</creatorcontrib><creatorcontrib>KORNELIA TITZE</creatorcontrib><creatorcontrib>VOLKER MARSCHNER</creatorcontrib><creatorcontrib>SILVIA PALADINO</creatorcontrib><creatorcontrib>PATRICK LORENZ</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>TATIANA SCHWARZ</au><au>QUOC KHANH NGUYEN</au><au>KORNELIA TITZE</au><au>VOLKER MARSCHNER</au><au>SILVIA PALADINO</au><au>PATRICK LORENZ</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>CLONING, EXPRESSION AND USE OF ACID PHOSPHOLIPASES</title><date>2012-05-29</date><risdate>2012</risdate><abstract>The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID No: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for with the restriction map by the pPL3940-Topo2.5 plasmid according to figure 7 and that are stored under number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) in the basis of the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: a or a sequence derived therefrom, which can be obtained by substituting, adding, or deleting one or more amino acids, c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide for which a nucleic acid sequence codes, which is hybridized under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acids, f) allelic variants for amino acid sequences a) to e).
La invención se refiere a una secuencia de ADN que codifica para un polipéptido que tiene actividad de fosfolipasa, esencialmente sin actividad de lipasa, caracterizado porque la secuencia de ADN es seleccionada de a) secuencias de ADN que contienen una secuencia de nucleótidos de conformidad con SEQ ID NO: 1, b) secuencias de ADN que contienen la secuencia codificadora de conformidad cori SEQ ID NO: 1, c) secuencias de ADN que codifican la secuencia de la proteína de conformidad con SEQ ID NO: 2, d) secuencias de ADN que son codificadas por el plásmido pPL3940-Topo2.5 con el mapa de restricción de conformidad con la Figura 7, el cual está depositado bajo el número de acceso DSM 22741, e) secuencias de ADN que hibridan bajo condiciones astringentes con una de las secuencias de ADN de conformidad con a), b), c) o d), f) secuencias de ADN que están relacionadas con las secuencias de ADN de conformidad con a), b), c), d) o e) debido a la degeneración del código genético, y g) cadenas complementarias a las secuencias de conformidad con a) a f), caracterizadas porque la secuencia de ADN preferentemente es derivada de Aspergillus y más preferiblemente de Aspergillus fumigalzus, y un polipéptido que tiene actividad de fosfolipasa, esencialmente sin actividad de lipasa seleccionado de a) un polipéptido que es codificado por la parte codificadora de una secuencia de ADN como se ha definido anteriormente, b) un polipéptido que tiene la secuencia de conformidad con SEQ ID NO: 2 o una secuencia derivada de ésta, la cual puede ser obtenida por substitución adición, deleción de uno o más aminoácidos, c) un polipéptido que tiene una secuencia que tiene al menos 83% de identidad con los aminoácidos 1 a 299 de SEQ ID NO: 2, d) un polipéptido que es codificado por una secuencia de ácido nucleico que hibrida bajo condiciones astringentes con (i) los nucleótidos 55 a 1106 de SEQ ID NO: 1, (ii) la secuencia de ADNC contenida en los nucleótidos 55 a 1106 de SEQ ID NO: 1, (iii) una secuencia parcial de (1) o (ii) compuesta por al menos 100 nucleótidos, o (iv) una cadena complementaria de (i), (ii) o (iii), e) una variante del polipéptido que tiene SEQ ID NO: 2 conteniendo una substitución, deleción y/o inserción de uno o más aminoácidos, f) variantes alélicas de las secuencias de aminoácidos a) a e).</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng ; spa |
| recordid | cdi_epo_espacenet_MX2012004725A |
| source | esp@cenet |
| subjects | ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY BLEACHING LEATHER OR FURS CANDLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DETERGENTS DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS,YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS ENZYMOLOGY ESSENTIAL OILS FATTY ACIDS THEREFROM FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR LAUNDERING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFUMES PRODUCING (PRESSING, EXTRACTION), REFINING AND PRESERVINGFATS, FATTY SUBSTANCES (e.g. LANOLIN), FATTY OILS AND WAXES,INCLUDING EXTRACTION FROM WASTE MATERIALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TEXTILES TREATMENT OF TEXTILES OR THE LIKE VINEGAR WINE |
| title | CLONING, EXPRESSION AND USE OF ACID PHOSPHOLIPASES |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T03%3A58%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=TATIANA%20SCHWARZ&rft.date=2012-05-29&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EMX2012004725A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |