CLONING, EXPRESSION AND USE OF ACID PHOSPHOLIPASES

The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID No: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for with the restriction map by the pPL3940-Topo2.5 plasmid according to figure 7 and that are stored under number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) in the basis of the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: a or a sequence derived therefrom, which can be obtained by substituting, adding, or deleting one or more amino acids, c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide for which a nucleic acid sequence codes, which is hybridized under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acids, f) allelic variants for amino acid sequences a) to e). La invención se refiere a una secuencia de ADN que codifica para un polipéptido que tiene actividad de fosfolipasa, esencialmente sin actividad de lipasa, caracterizado porque la secuencia de ADN es seleccionada de a) secuencias de ADN que contienen una secuencia de nucleótidos de conformidad con SEQ ID NO: 1, b) secuencias de ADN que contienen la secuencia codificadora de conformidad cori SEQ ID NO: 1, c) secuencias de ADN que c