CHIMERIC PORCINE CIRCOVIRUS PCV2Gen-1Rep AND USES THEREOF

The present invention relates to a novel chimeric nucleic acid molecule of porcine circovirus (PCV2Gen-lRep) that embraces a nucleic acid molecule encoding porcine circovirus type 2 (PCV2) which contains a nucleic acid sequence encoding a Rep protein of porcine circovirus type 1 (PCVl), particularly wherein the nucleic acid sequence encoding the Rep protein of PCVl is an open reading frame (ORF) gene and, more particularly, wherein the ORF Rep gene is ORFl. A highly desirable chimeric nucleic acid molecule is constructed by replacing the ORFl Rep gene of PCV2 by the ORFl Rep gene of PCVl. The invention also encompasses the biologically functional plasmid or viral vector containing the unique chimeric nucleic acid molecules, suitable host cells transfected by the plasmid or vector, infectious chimeric porcine circoviruses that are produced by the suitable host cells, the process for the production of an immunogenic polypeptide product making use of the new chimera, viral vaccines that protect a pig against viral infection or postweaning multisystemic wasting syndrome (PMWS) caused by PCV2, methods of protecting a pig against viral infection or postweaning multisystemic wasting syndrome (PMWS) caused by PCV2, methods of preparing the unique chimera of PCV2Gen-lRep and the like. This invention further includes a new method for improving the replication and titer of PCV2 in a cell culture. La presente invención se refiere a una molécula de ácido nucleico quimérico novedoso del circovirus porcino (PCV2Gen-1 Rep) que comprende una molécula de ácido nucleico que codifica el circovirus porcino de tipo 2 (PCV2), que contiene una secuencia de ácido nucleico que codifica una proteína Rep del circovirus porcino de tipo 1 (PCV1), particularmente en donde la secuencia de ácido nucleico que codifica la proteína Rep de PCVI es un gen del marco de lectura abierto (ORF) y, más particularmente, en donde el gen Rep del ORF es ORF1; se construye una molécula de ácido nucleico quimérico muy conveniente reemplazando el gen Rep de ORF1 de PCV2 con el gen Rep de ORF1 de PCV1; la invención también abarca el plásmido o vector viral biológicamente funcional que contiene las moléculas de ácido nucleico quimérico único, células hospederas adecuadas transfectadas por el plásmido o vector, circovirus porcinos quiméricos infecciosos que son producidos por las células hospederas adecuadas, el procedimiento de producción de un producto polipeptídico inmunogénico que utiliza la quimera nueva