UNITARY OPERATION FOR THE SPREAD OF MAMMAL CELLS USING A NOVEL CULTURE MEDIUM FOR ASSESSING THE BIOLOGICAL ACTIVITY AND PRODUCTION OF COMPOUNDS OF BIOTECHNOLOGICAL MATTER

Described is a unitary operation for the spread of mammal cells of the lines OK (opossum kidney), CHANG (Human liver) and LLC-PK1 (pig kidney), which allows said three cell lines to be a 100% adapted by means of a novel culture medium MCR; the MEM medium was used for the adaptation in MCR medium and as a reference for evaluating the growth parameters of the aforesaid cell lines, such as: growth kinetics, morphologic analysis by light microscopy and cytotoxicity analysis with HgC12, also the protein total concentration and the protein pattern in polyacrylamide gels and criopreservation of the cells was performed. Cell viability was performed by trypan blue. According to the results obtained in the growth kinetics, the light microscopy morphology, the sensibility tests before the HgC12, the protein concentration, the cells propagated both in MEM and MCR were similar and there were not significant differences (p