INSECTICIDAL COMPOSITIONS AND METHODS FOR MAKING INSECT-RESISTANT TRANSGENIC PLANTS

The present invention provides isolated polynucleotide sequences encoding ET37, TIC810 and TIC812 proteins from Bacillus thuringiensis, and nucleotide sequences for use in expressing TIC809, ET37, TIC810 and TIC812, and fusions of various insecticidally effective combinations of these proteins such...

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Bibliographische Detailangaben
Hauptverfasser:	ANDERSON, HEATHER M, ROBERTS, JAMES K, ZHANG, BEI, CHAY, CATHERINE A, BAUM, JAMES A
Format:	Patent
Sprache:	eng ; spa
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides isolated polynucleotide sequences encoding ET37, TIC810 and TIC812 proteins from Bacillus thuringiensis, and nucleotide sequences for use in expressing TIC809, ET37, TIC810 and TIC812, and fusions of various insecticidally effective combinations of these proteins such as TIC 127, in plants. Methods of making and using the polynucleotide sequences and the proteins in the development of transgenic plant cells and transgenic plants exhibiting improved insect resistance against (1) Coleopteran insects including Western Corn Rootworm (Dibrotica virgifera), Southern Corn Rootworm (Dibrotica undecempunctata), Northern Corn Rootworm (Diabrotica barbed), Mexican Corn Rootworm (Diabrotica virgifera zeae), Brazilian Corn Rootworm (Diabrotica balteata) andBrazilian Corn Rootworm complex (Diabrotica viridula and Diabrotica speciosa), and against Hemipteran insects such as Lygus bugs, are disclosed. La presente invencion provee secuencias de polinucleotidos aisladas que codifican las proteinas ET37, TIC81O y T1C812 a partir de BacilIus thuringiensis, y secuencias de nucleotidos para usar en la expresion de T1C809, ET37, TIC81O y T1C812, y fusiones de diversas combinaciones de estas proteinas efectivas desde el punto de vista insecticida tales como TIC 127, en plantas; se describen metodos para hacer y usar las secuencias de polinucleotidos y las proteinas en el desarrollo de celulas de plantas transgenicas y plantas transgenicas que presentan una resistencia a insectos mejorada contra (1) insectos coleopteros incluyendo el gusano de raiz del maiz occidental (Dibrotica virgifera), gusano de raiz del maiz del sur (Dibrotica undecempunctata), gusano de raiz del maiz del norte (Diabrotica barbeci), gusano de raiz del maiz mexicano (Diabrotica virgifera zeae), gusano de raiz del maiz brasileno (Diabrotica balteata) y complejo de gusano de raiz del maiz brasileno (Diabrotica viridula y Diabrotica speciosa), y contra insectos hemipteros tales como chinches Lygus.