Method for submerged cultivation of Lentinus edodes (Berk.) Sing. CNMN-FB-01 fungi strain

The invention relates to biotechnology, namely to the submerged cultivation of Lentinus edodes (Berk.) Sing. CNMN-FB-01 fungi strain, producer of biomass that can be used for producing medicinal preparations with curative and nutraceutical properties.The method for submerged cultivation of Lentinus...

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Hauptverfasser: DVORNINA Elena, RUDIC Valeriu, BULHAC Ion, URECHE Dumitru, CILOCI Alexandra, COCU Maria
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creator DVORNINA Elena
RUDIC Valeriu
BULHAC Ion
URECHE Dumitru
CILOCI Alexandra
COCU Maria
description The invention relates to biotechnology, namely to the submerged cultivation of Lentinus edodes (Berk.) Sing. CNMN-FB-01 fungi strain, producer of biomass that can be used for producing medicinal preparations with curative and nutraceutical properties.The method for submerged cultivation of Lentinus edodes (Berk.) Sing. CNMN-FB-01 fungi strain provides for the inoculation of seed material in the amount of 10% v/v into a nutrient medium, containing, g/L: NH4NO3 0.20, KH2PO4 1.30, MgSO4·7H2O 0.35, tris(2,6-dimethyl pyridinedicarboxylate-1kONO)-di-µ-(isothiocyanato-1.2kN)-(diisocyanato-2kN)barium(II)cobalt(II) 0.005-0.015, beer wort 5°Balling the rest, and cultivation with continuous stirring at a temperature of 28-30°C for 144 hours.The technical result of the invention consists in reducing the duration of cultivation by 48 hours and increasing the production of biomass. Invenţia se referă la biotehnologie, şi anume la cultivarea submersă a tulpinii de fungi Lentinus edodes (Berk.) Sing. CNMN-FB-01, producătoare de biomasă care poate fi utilizată pentru fabricarea preparatelor medicamentoase cu proprietăţi curative şi nutraceutice.Procedeul de cultivare submersă a tulpinii de fungi Lentinus edodes (Berk) Sing CNMN-FB-01 prevede inocularea materialului semincer în cantitate de 10% v/v pe mediul nutritiv, care conţine, g/L: NH4NO3 0,20, KH2PO4 1,30, MgSO4·7H2O 0,35, tris(2,6-dimetil piridindicarboxilat-1kONO)-di-µ-(izotiocianato-1,2kN)-(diizotiocianato-2kN)bariu(II)cobalt(II) 0,005-0,015, malţ de 5° Balling restul, şi cultivarea la agitare continuă la temperatura de 28-30°C în decurs de 144 de ore.Rezultatul tehnic al invenţiei constă în reducerea ciclului de cultivare cu 48 de ore şi sporirea producţiei de biomasă. Изобретение относится к биотехнологии, а именно к глубинному культивированию штамма грибов Lentinus edodes (Berk.) Sing. CNMN-FB-01, производителя биомассы, которая может быть использована для получения лекарственных препаратов с лечебными и нутрацевтическими сойствами.Способ глубинного культивирования штамма грибов Lentinus edodes (Berk.) Sing. CNMN-FB-01 предусматривает инокуляцию семенного материала в количестве 10% об./об. в питательную среду, которая содержит, г/л: NH4NO3 0,20, KH2PO4 1,30, MgSO4·7H2O 0,35, трис(2,6-диметил пиридиндикарбоксилат-1kONO)-ди-µ-(изотиоцианато-1,2kN)-(диизоцианато-2kN)барий(II)кобальт(II) 0,005-0,015, пивное сусло 5°Баллинга остальное, и культивирование при непрерывном перемешивании при температуре 28-30°Cв течение 1
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CNMN-FB-01 fungi strain</title><source>esp@cenet</source><creator>DVORNINA Elena ; RUDIC Valeriu ; BULHAC Ion ; URECHE Dumitru ; CILOCI Alexandra ; COCU Maria</creator><creatorcontrib>DVORNINA Elena ; RUDIC Valeriu ; BULHAC Ion ; URECHE Dumitru ; CILOCI Alexandra ; COCU Maria</creatorcontrib><description>The invention relates to biotechnology, namely to the submerged cultivation of Lentinus edodes (Berk.) Sing. CNMN-FB-01 fungi strain, producer of biomass that can be used for producing medicinal preparations with curative and nutraceutical properties.The method for submerged cultivation of Lentinus edodes (Berk.) Sing. CNMN-FB-01 fungi strain provides for the inoculation of seed material in the amount of 10% v/v into a nutrient medium, containing, g/L: NH4NO3 0.20, KH2PO4 1.30, MgSO4·7H2O 0.35, tris(2,6-dimethyl pyridinedicarboxylate-1kONO)-di-µ-(isothiocyanato-1.2kN)-(diisocyanato-2kN)barium(II)cobalt(II) 0.005-0.015, beer wort 5°Balling the rest, and cultivation with continuous stirring at a temperature of 28-30°C for 144 hours.The technical result of the invention consists in reducing the duration of cultivation by 48 hours and increasing the production of biomass. Invenţia se referă la biotehnologie, şi anume la cultivarea submersă a tulpinii de fungi Lentinus edodes (Berk.) Sing. CNMN-FB-01, producătoare de biomasă care poate fi utilizată pentru fabricarea preparatelor medicamentoase cu proprietăţi curative şi nutraceutice.Procedeul de cultivare submersă a tulpinii de fungi Lentinus edodes (Berk) Sing CNMN-FB-01 prevede inocularea materialului semincer în cantitate de 10% v/v pe mediul nutritiv, care conţine, g/L: NH4NO3 0,20, KH2PO4 1,30, MgSO4·7H2O 0,35, tris(2,6-dimetil piridindicarboxilat-1kONO)-di-µ-(izotiocianato-1,2kN)-(diizotiocianato-2kN)bariu(II)cobalt(II) 0,005-0,015, malţ de 5° Balling restul, şi cultivarea la agitare continuă la temperatura de 28-30°C în decurs de 144 de ore.Rezultatul tehnic al invenţiei constă în reducerea ciclului de cultivare cu 48 de ore şi sporirea producţiei de biomasă. Изобретение относится к биотехнологии, а именно к глубинному культивированию штамма грибов Lentinus edodes (Berk.) Sing. CNMN-FB-01, производителя биомассы, которая может быть использована для получения лекарственных препаратов с лечебными и нутрацевтическими сойствами.Способ глубинного культивирования штамма грибов Lentinus edodes (Berk.) Sing. 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Sing. CNMN-FB-01 fungi strain</title><description>The invention relates to biotechnology, namely to the submerged cultivation of Lentinus edodes (Berk.) Sing. CNMN-FB-01 fungi strain, producer of biomass that can be used for producing medicinal preparations with curative and nutraceutical properties.The method for submerged cultivation of Lentinus edodes (Berk.) Sing. CNMN-FB-01 fungi strain provides for the inoculation of seed material in the amount of 10% v/v into a nutrient medium, containing, g/L: NH4NO3 0.20, KH2PO4 1.30, MgSO4·7H2O 0.35, tris(2,6-dimethyl pyridinedicarboxylate-1kONO)-di-µ-(isothiocyanato-1.2kN)-(diisocyanato-2kN)barium(II)cobalt(II) 0.005-0.015, beer wort 5°Balling the rest, and cultivation with continuous stirring at a temperature of 28-30°C for 144 hours.The technical result of the invention consists in reducing the duration of cultivation by 48 hours and increasing the production of biomass. Invenţia se referă la biotehnologie, şi anume la cultivarea submersă a tulpinii de fungi Lentinus edodes (Berk.) Sing. CNMN-FB-01, producătoare de biomasă care poate fi utilizată pentru fabricarea preparatelor medicamentoase cu proprietăţi curative şi nutraceutice.Procedeul de cultivare submersă a tulpinii de fungi Lentinus edodes (Berk) Sing CNMN-FB-01 prevede inocularea materialului semincer în cantitate de 10% v/v pe mediul nutritiv, care conţine, g/L: NH4NO3 0,20, KH2PO4 1,30, MgSO4·7H2O 0,35, tris(2,6-dimetil piridindicarboxilat-1kONO)-di-µ-(izotiocianato-1,2kN)-(diizotiocianato-2kN)bariu(II)cobalt(II) 0,005-0,015, malţ de 5° Balling restul, şi cultivarea la agitare continuă la temperatura de 28-30°C în decurs de 144 de ore.Rezultatul tehnic al invenţiei constă în reducerea ciclului de cultivare cu 48 de ore şi sporirea producţiei de biomasă. Изобретение относится к биотехнологии, а именно к глубинному культивированию штамма грибов Lentinus edodes (Berk.) Sing. CNMN-FB-01, производителя биомассы, которая может быть использована для получения лекарственных препаратов с лечебными и нутрацевтическими сойствами.Способ глубинного культивирования штамма грибов Lentinus edodes (Berk.) Sing. CNMN-FB-01 предусматривает инокуляцию семенного материала в количестве 10% об./об. в питательную среду, которая содержит, г/л: NH4NO3 0,20, KH2PO4 1,30, MgSO4·7H2O 0,35, трис(2,6-диметил пиридиндикарбоксилат-1kONO)-ди-µ-(изотиоцианато-1,2kN)-(диизоцианато-2kN)барий(II)кобальт(II) 0,005-0,015, пивное сусло 5°Баллинга остальное, и культивирование при непрерывном перемешивании при температуре 28-30°Cв течение 144 часов.Технический результат изобретения состоит в сокращении продолжительности культивирования на 48 часов и повышении производства биомассы.</description><subject>ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM</subject><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HETEROCYCLIC COMPOUNDS</subject><subject>HORTICULTURE</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WATERING</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqFyasOwjAUANAaBAE-geRKEF0gm5heYUHQGTCopVtvS8O4Xfrg-zF41BFnyR4S09NrMD5AzMMbg0UNY56S-6jkPIE3cEVKjnIE1F5jhF2D4VXs4ebIFiA62fG24YcjmEzWQUxBOVqzhVFTxM3PFdu257u4cJx9j3FWIxKmXp6quipFXf77LyRGNqA</recordid><startdate>20240229</startdate><enddate>20240229</enddate><creator>DVORNINA Elena</creator><creator>RUDIC Valeriu</creator><creator>BULHAC Ion</creator><creator>URECHE Dumitru</creator><creator>CILOCI Alexandra</creator><creator>COCU Maria</creator><scope>EVB</scope></search><sort><creationdate>20240229</creationdate><title>Method for submerged cultivation of Lentinus edodes (Berk.) 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Sing. CNMN-FB-01 fungi strain</title><date>2024-02-29</date><risdate>2024</risdate><abstract>The invention relates to biotechnology, namely to the submerged cultivation of Lentinus edodes (Berk.) Sing. CNMN-FB-01 fungi strain, producer of biomass that can be used for producing medicinal preparations with curative and nutraceutical properties.The method for submerged cultivation of Lentinus edodes (Berk.) Sing. CNMN-FB-01 fungi strain provides for the inoculation of seed material in the amount of 10% v/v into a nutrient medium, containing, g/L: NH4NO3 0.20, KH2PO4 1.30, MgSO4·7H2O 0.35, tris(2,6-dimethyl pyridinedicarboxylate-1kONO)-di-µ-(isothiocyanato-1.2kN)-(diisocyanato-2kN)barium(II)cobalt(II) 0.005-0.015, beer wort 5°Balling the rest, and cultivation with continuous stirring at a temperature of 28-30°C for 144 hours.The technical result of the invention consists in reducing the duration of cultivation by 48 hours and increasing the production of biomass. Invenţia se referă la biotehnologie, şi anume la cultivarea submersă a tulpinii de fungi Lentinus edodes (Berk.) Sing. CNMN-FB-01, producătoare de biomasă care poate fi utilizată pentru fabricarea preparatelor medicamentoase cu proprietăţi curative şi nutraceutice.Procedeul de cultivare submersă a tulpinii de fungi Lentinus edodes (Berk) Sing CNMN-FB-01 prevede inocularea materialului semincer în cantitate de 10% v/v pe mediul nutritiv, care conţine, g/L: NH4NO3 0,20, KH2PO4 1,30, MgSO4·7H2O 0,35, tris(2,6-dimetil piridindicarboxilat-1kONO)-di-µ-(izotiocianato-1,2kN)-(diizotiocianato-2kN)bariu(II)cobalt(II) 0,005-0,015, malţ de 5° Balling restul, şi cultivarea la agitare continuă la temperatura de 28-30°C în decurs de 144 de ore.Rezultatul tehnic al invenţiei constă în reducerea ciclului de cultivare cu 48 de ore şi sporirea producţiei de biomasă. Изобретение относится к биотехнологии, а именно к глубинному культивированию штамма грибов Lentinus edodes (Berk.) Sing. CNMN-FB-01, производителя биомассы, которая может быть использована для получения лекарственных препаратов с лечебными и нутрацевтическими сойствами.Способ глубинного культивирования штамма грибов Lentinus edodes (Berk.) Sing. CNMN-FB-01 предусматривает инокуляцию семенного материала в количестве 10% об./об. в питательную среду, которая содержит, г/л: NH4NO3 0,20, KH2PO4 1,30, MgSO4·7H2O 0,35, трис(2,6-диметил пиридиндикарбоксилат-1kONO)-ди-µ-(изотиоцианато-1,2kN)-(диизоцианато-2kN)барий(II)кобальт(II) 0,005-0,015, пивное сусло 5°Баллинга остальное, и культивирование при непрерывном перемешивании при температуре 28-30°Cв течение 144 часов.Технический результат изобретения состоит в сокращении продолжительности культивирования на 48 часов и повышении производства биомассы.</abstract><oa>free_for_read</oa></addata></record>
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subjects ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAININGELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN,SULFUR, SELENIUM OR TELLURIUM
AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HETEROCYCLIC COMPOUNDS
HORTICULTURE
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TRAPPING
VINEGAR
WATERING
WINE
title Method for submerged cultivation of Lentinus edodes (Berk.) Sing. CNMN-FB-01 fungi strain
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