Method for determining the activity of catalase

The invention relates to biotechnology, in particular to a method for determining the activity of catalase and can be used in biochemical research, as well as to control the technological process of catalase preparation in the microbiological industry.Summary of the method consists in that the biological material is introduced into a mixture containing: 0.05 M of phosphate buffer, 0.06% of hydrogen peroxide and 5 mM of ethylene diamine tetraacetic acid, then the mixture is incubated at the temperature of 25°C and is determined spectrophotometrically the optical density of the reaction product in relation to phosphate buffer solution, the measurements are performed at a wavelength of 240 nm at the beginning of the reaction and in 15 seconds, and the activity of catalase is defined in accordance with the established formula. Inventia se refera la biotehnologie, în special la o metoda de determinare a activitatii catalazei si poate fi utilizata în cercetari biochimice, precum si pentru controlul procesului tehnologic de obtinere a preparatului catalazei în industria microbiologica.Esenta metodei consta în aceea ca materialul biologic se introduce într-un amestec ce contine: 0,05 M de tampon fosfat, 0,06% de peroxid de hidrogen si 5 mM de acid etilendiamintetraacetic, apoi amestecul se incubeaza la temperatura de 25ºC si se determina spectrofotometric densitatea optica a produsului reactiei în raport cu solutia de tampon fosfat, masurarile se efectueaza la lungimea de unda de 240 nm la începutul reactiei si peste 15 secunde, iar activitatea catalazei se determina conform unei formule stabilite.