Process for obtaining of tetraoside [(25R)- 5?-furostan-3?,22?,26-triol]-26-O-?-D-glucopyranoside
The invention refers to the bio-organic chemistry, in particular to a process for obtaining of tetraoside [(25R)-5?-furostan-3?,22?,26-triol]-26-O-?-D-glucopyranoside, manifesting gibberellenic activity. The process includes degreasing of Petunia hybrida hort. seeds, obtaining of extract with hydroa...
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| Format: | Patent |
| Sprache: | eng ; rum |
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| Zusammenfassung: | The invention refers to the bio-organic chemistry, in particular to a process for obtaining of tetraoside [(25R)-5?-furostan-3?,22?,26-triol]-26-O-?-D-glucopyranoside, manifesting gibberellenic activity. The process includes degreasing of Petunia hybrida hort. seeds, obtaining of extract with hydroalcoholic solution, concentration of the extract, silica gel column chromatography of the obtained residue, using in the capacity of eluent the chloroform-methanol-water system in the ratio of 60:30:10...65:35:10, acetylation of the steroid glycoside fractions with acetic anhydride in pyridine, chromatography of the obtained derivatives onto a column with silica gel impregnated with 15% AgNO3 aqueous solution in the chloroform-ethanol eluents system in the ratio of 3:1...45:46 and saponification of the separated peracetate with the subsequent isolation of the final product.Claims: 1 |
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