Process for the cultivation of cyanobacterium Spirulina platensis

The invention refers to phycobiotechnology, in particular to a process for the cultivation of cyanobacterium Spirulina platensis with an increased content of phycobiliproteins that can be used in the pharmaceutical industry and medicine.The process for the cultivation of cyanobacterium Spirulina pla...

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Hauptverfasser: ŢAPCOV Victor, ELENCIUC Daniela, RUDIC Valeriu, BULIMAGA Valentina, BATÎR Ludmila, GULEA Aurelian, BEJENARI Natalia, ZOSIM Liliana
Format: Patent
Sprache:eng ; rum ; rus
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Zusammenfassung:The invention refers to phycobiotechnology, in particular to a process for the cultivation of cyanobacterium Spirulina platensis with an increased content of phycobiliproteins that can be used in the pharmaceutical industry and medicine.The process for the cultivation of cyanobacterium Spirulina platensis includes cultivation on a nutrient medium, containing, g/L: NaHCO3 - 16.8; K2HPO4·3H2O - 1.0; NaNO3 - 2.5; NaCl - 1.0; K2SO4 - 1.0; K2SO4 - 1.0; CaCl2·6H2O - 0.04; MgSO4·7H2O - 0.20; H3BO3 - 0.00286; MnCl2·4H2O - 0.00181; ZnSO4·7H2O - 0.00022; CuSO4·5H2O - 0.00008; MoO3 - 0.000015; 1 mL of 0.09 M FeHEDTA solution and distilled water up to 1 L. On the second day of cultivation in the said medium is added 3...4 mg/L of nitrate hydrate of 5-nitrosalicylidenethiosemicarbazido(1-)aquacopper(II). The cultivation is carried out during 6 days, at a temperature of 30...35°C, at the illumination of 2000...3000 lx in the first two days and of 3000...4000 lx in the following days. Invenţia se referă la ficobiotehnologie, în particular la un procedeu de cultivare a cianobacteriei Spirulina platensis cu un conţinut sporit de ficobiliproteine, care pot fi utilizate în industria farmaceutică şi medicină.Procedeul de cultivare a cianobacteriei Spirulina platensis include cultivarea pe un mediu nutritiv, conţinând, g/L: NaHCO3 - 16,8; K2HPO4·3H2O - 1,0; NaNO3 - 2,5; NaCl - 1,0; K2SO4 - 1,0; CaCl2·6H2O - 0,04; MgSO4·7H2O - 0,20; H3BO3 - 0,00286; MnCl2·4H2O - 0,00181; ZnSO4·7H2O - 0,00022; CuSO4·5H2O - 0,00008; MoO3 - 0,000015; 1mL de soluţie FeHEDTA de 0,09 M, apă distilată până la 1 L. La a 2-a zi de cultivare în mediu se adaugă 3...4 mg/L de hidrat al nitratului de 5-nitrosalicilidentiosemicarbazido(1-)aquacupru(II). Cultivarea se efectuează timp de 6 zile, la temperatura de 30 ... 35°C, la iluminarea de 2000...3000 lx în primele 2 zile şi de 3000...4000 lx în următoarele zile. Изобретение относится к фикобиотехнологии, в частности к способу культивирования цианобактерии Spirulina platensis с высоким содержанием фикобилипротеинов, которые могут быть использованы в фармацевтической промышленности и в медицине.Способ культивирования цианобактерии Spirulina platensis включает культивирование на питательной среде, содержащей, г/л: NaHCO3 - 16,8; K2HPO4·3H2O - 1,0; NaNO3 - 2,5; NaCl - 1,0; K2SO4 - 1,0; CaCl2·6H2O - 0,04; MgSO4·7H2O - 0,20; H3BO3 - 0,00286; MnCl2·4H2O - 0,00181; ZnSO4·7H2O - 0,00022; CuSO4·5H2O - 0,00008; MoO3 - 0,000015; 1 мл 0,09 М раствора FeHEDTA и дистилли