Process for preserving the Saccharomyces cerevisiae CNMN-Y-20 yeast strain
The invention relates to biotechnology, in particular to a process for preserving the Saccharomyces cerevisiae CNMN-Y-20 yeast strain, which can be used for long-term storage of microorganisms and their use as sources of biologically active substances.The process, according to the invention, consist...
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Zusammenfassung: | The invention relates to biotechnology, in particular to a process for preserving the Saccharomyces cerevisiae CNMN-Y-20 yeast strain, which can be used for long-term storage of microorganisms and their use as sources of biologically active substances.The process, according to the invention, consists in cultivating the Saccharomyces cerevisiae CNMN-Y-20 yeast strain on a nutrient medium for 72 hours at a temperature of 26...28°C, suspending the culture in a medium consisting of skim milk and 5...10% vol. solution comprising 10 mg/ml of extract of sulfated polysaccharides, obtained from spirulina biomass, to a titer of 105...106 ml-1, rapid freezing at a temperature of -20°C, lyophilization and storage at a temperature of 4°C.The technical result of the invention consists in increasing the protein content in the Saccharomyces cerevisiae CNMN-Y-20 yeast biomass after one year of storage in a lyophilized state by 25.32...26.62%.
Invenţia se referă la biotehnologie, şi anume la un procedeu de conservare a tulpinii de levuri Saccharomyces cerevisiae CNMN-Y-20, care poate fi utilizat pentru păstrarea microorganismelor pe un termen îndelungat şi utilizarea acestora ca surse de substanţe biologic active.Procedeul, conform invenţiei, constă în cultivarea tulpinii de levuri Saccharomyces cerevisiae CNMN-Y-20 pe un mediu nutritiv în decurs de 72 de ore la temperatura de 26...28°C, suspensionarea culturii într-un mediu constituit din lapte degresat şi 5...10% vol. soluţie ce conţine 10 mg/ml de extract de polizaharide sulfatate, obţinut din biomasa de spirulină, până la titrul de 105...106 ml-1, congelarea rapidă la temperatura de -20°C, liofilizarea şi păstrarea la temperatura de 4°C.Rezultatul tehnic al invenţiei constă în majorarea conţinutului de proteine în biomasa de levuri Saccharomyces cerevisiae CNMN-Y-20 după un an de păstrare în stare liofilizată cu 25,32...26,62%.
Изобретение относится к биотехнологии, а именно к способу консервирования штамма дрожжей Saccharomyces cerevisiae CNMN-Y-20, который может быть использован для долговременного хранения микроорганизмов и их использования в качестве источников биологически активных веществ.Способ, согласно изобретению, заключается в культивировании штамма Saccharomyces cerevisiae CNMN-Y-20 на питательной среде в течение 72 часов при температуре 26...28°C, суспендировании культуры в среде состоящей из обезжиренного молока и 5...10%об. раствора содержащего 10 мг/мл экстракта сульфатированных полисахаридов, полученног |
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