Fermentativs panemiens 7beta-(4-karboksibutan-amid)cefalosporanskabes iegusanai izmantojot modificetu trigonopsis variabilis fermentu d-aminoskabju oksidazi kas produceta escherichia coli

Enzymatic process for the preparation of cephalosporanic 7beta-(4-carboxybutanamide) acid by using the modified enzyme D-aminoacid oxidase of Trigonopsis variabilis produced in Escherichia coli . The process for the expression of the enzyme comprises: (I) isolating the DNA corresponding to the gene...

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Hauptverfasser:	JORGE ALONSO PALACIOS, JOSE LUIS GARCIA LOPEZ, ESTRELLA CORTES RUBIO, BRUNO DIEZ GARCIA, FRANCISCO SALTO MALDONADO, ENCARNACION MELLADO DURAN, JOSE LUIS BARREDO FUENTE, JOSE MANUEL GUISAN SEIJAS
Format:	Patent
Sprache:	lav
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	Enzymatic process for the preparation of cephalosporanic 7beta-(4-carboxybutanamide) acid by using the modified enzyme D-aminoacid oxidase of Trigonopsis variabilis produced in Escherichia coli . The process for the expression of the enzyme comprises: (I) isolating the DNA corresponding to the gene which codes for the enzyme D-aminoacide oxidase; (II) removing the intron which is contained in said gene; (III) inserting the DNA fragment obtained into the plasmide which is capable of replication in Escherichia coli ; (IV) fusing at the extremity 5' of the structural region of the gene a synthetic assembler which contains a nucleotide sequence which codes for six histidines; (V) transforming a strain of Escherichia coli with the resulting recombinant plasmide; (VI) cultivating the transformed cells of Escherichia coli ; and (VII) recovering the enzyme D-aminoacid oxidase of the former cultivation operation through affinity chromatography.