COMPOSITION DESTINEE A LA DETERMINATION DE LA TRI-IODO THYRONINE,SON PROCEDE DE PREPARATION,ET METHODE DE DIAGNOSTIQUE UTILISANT CETTE COMPOSITION

A composition for diagnostic use is disclosed, more particularly for the dosage of tri-iodo thyronine in human blood serum: the subject composition comprises tri-iode thyronine as such and an enzyme which is covalently bonded to tri-iodo thyronine. A diagnostic method is also disclosed, which is int...

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Hauptverfasser: BARONCELLI V, RENZI P
Format: Patent
Sprache:fre
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Zusammenfassung:A composition for diagnostic use is disclosed, more particularly for the dosage of tri-iodo thyronine in human blood serum: the subject composition comprises tri-iode thyronine as such and an enzyme which is covalently bonded to tri-iodo thyronine. A diagnostic method is also disclosed, which is intended for using the above specified composition in diagnostic runs. The method, briefly resumed, consists removing the tri-iodo thyronine of the serum sample being assayed by means of an insolubilized anti-T3 antibody and treating the residue with the sensibilized enzyme which is nothing else than the covalent formed by tri-iodo thyronine and an appropriate enzyme, such as carbonic anhydrase.