Transgenic Strain of Metarhizium anisopliae and Preparation Method and Application thereof

The invention discloses a transgenic strain of Metarhizium anisopliae, preparation method and application thereof, which is a recombinant strain constructed by Metarhizium anisopliae gene and exogenous subtilisin-like protease (SLP) Prla gene. The principle of Metarhizium anisopliae transgenic strai...

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Bibliographische Detailangaben
Hauptverfasser:	LIU Caixia, ZHAO Haizheng, LIU Baorui, JIANG Meng, ZHANG Fan, WANG Xuejun, ZANG Yanan
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a transgenic strain of Metarhizium anisopliae, preparation method and application thereof, which is a recombinant strain constructed by Metarhizium anisopliae gene and exogenous subtilisin-like protease (SLP) Prla gene. The principle of Metarhizium anisopliae transgenic strain is as follows: basing on Metarhizium anisopliae, and then using Pgpd as a strong promoter and TTrpC as a terminator as well as Basta as a screening marker; next, constructing a subtilisin-like protease Pr1al overexpression box by using a fusion PCR method, preparing fungal protoplasts, and then carrying out genetic engineering transformation by using a CaCl2-PEG method; finally, randomly inserting the overexpression box into the genome of Metarhizium anisopliae for overexpression. The Metarhizium anisopliae transgenic strain has some advantages of good killing effect on Blattella germanica, strong toxicity and effectively controlling Blattella germanica population in a short time.