METHOD FOR ESTABLISHING ANTI-NEW CORONAVIRUS DRUG SCREENING AND EVALUATION MODEL AND APPLICATION THEREOF

The present invention discloses a method for establishing an anti-new coronavirus drug screening and evaluation model and an application thereof. Two non-fluorescent complementary fragments VN155 and VC155 based on a fluorescent protein Venus are recombined to form a fluorescent protein to emit a fluorescent signal, and simultaneous expression of multiple genes is implemented by using a self-cleavage property of a 2A polypeptide, to detect a binding effect between a Spike protein in the new coronavirus and an ACE2 receptor, which can be used for screening and preclinical efficacy evaluation of anti-SARS-CoV-2 drugs capable of cutting off adsorption to and infection of human cells by the new coronavirus. The present invention has advantages of simplicity, rapidity, trace amount and intuition, with good reproducibility of results, strong specificity, high sensitivity, less interference factors, less false positive and false negative, and is suitable for research and development of new anti-SARS-CoV-2 drugs.