METHOD FOR PREPARING NOVEL CORONAVIRUS PNEUMONIA BIVALENT VACCINE

Disclosed is a method for preparing a novel coronavirus pneumonia bivalent vaccine, which includes amplifying 2019-nCoV targeted interference gene shRNA, digesting PCR product, constructing interference vector pSilencer-shRNA, transferring DH5a, constructing shuttle vector pDC312 -shRNA and co-transfection of HEK293 with adenovirus backbone plasmid pBHGloxAEl to obtain Ad-shRNA, and then to obtain Ad-nCoVdsRNA; also includes amplifying 2019-nCoV antibody expression gene, digesting PCR product, constructing shuttle plasmid pShuttle-nCoV and transferring with DH5a, after PCR amplification, PCR co-transfecting HEK293 with adenovirus backbone plasmid pAd-nCoV to prepare recombinant adenovirus Ad-nCoV, and then prepare Ad-nCoVDNA; preparing a bivalent vaccine with Ad-nCoVdsRNA: Ad-nCoVDNA: H2O = 1: 1: 19; after inoculation via respiratory spray, Ad introduces nCoVdsRNA and nCoVDNA into cells, where nCoVdsRNA immediately generates dsRNA through its shRNA, and degrades homologous viral mRNA immediately, and nCoVDNA expresses proteins by encoding mRNA, and then produces antibodies to neutralize the virus.