A TRULY UNBIASED IN VITRO ASSAY TO PROFILE OFF-TARGET ACTIVITY OF ONE OR MORE TARGET-SPECIFIC PROGRAMMABLE NUCLEASES IN CELLS (ABNOBA-SEQ)

The present invention relates to a method for detecting off-target sites of one or more target-specific programmable nucleases in a genome in vitro, which allows for the detection of off-target sites in an input sample of less than 1 µg of genomic DNA. The present invention also relates to a method...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	HAAS, Simone Alexandra, HILDENBEUTEL, Markus, CATHOMEN, Toni, ANDRIEUX, Geoffroy, BÖRRIES, Melanie, MUSSOLINO, Claudio
Format:	Patent
Sprache:	eng ; lit
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The present invention relates to a method for detecting off-target sites of one or more target-specific programmable nucleases in a genome in vitro, which allows for the detection of off-target sites in an input sample of less than 1 µg of genomic DNA. The present invention also relates to a method for detecting in vivo off-target sites by using the in vitro method for detecting off-target sites of one or more target-specific programmable nucleases in a genome.