WHEP A PENTAMER-BASED FUSION PROTEIN COMPRISING A WHEP DOMAIN AND A SYSTEM FOR EXPRESSING THE SAME

The present invention relates to a fusion protein for improving the expression efficiency of a target protein. More specifically, it relates to a fusion protein produced by combining a pentameric toxin protein and a WHEP domain of hEPRS (Glutamyl-prolyl-tRNA synthetase from human) (including a linke...

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Bibliographische Detailangaben
Hauptverfasser:	KIM SUNG JAE, SEONG BAIK LIN, KIM DO DA, KIM SEUNGHOO
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a fusion protein for improving the expression efficiency of a target protein. More specifically, it relates to a fusion protein produced by combining a pentameric toxin protein and a WHEP domain of hEPRS (Glutamyl-prolyl-tRNA synthetase from human) (including a linker connecting the three domains and WHEP domains TRS-1, TRS-2, and TRS-3 located in the middle part of the EPRS protein) to a target protein, and the water solubility of the target protein is improved when the WHEP domain of hEPRS according to the present invention is used as a fusion partner for the expression of the target protein in E. coli. 본 발명은 목적 단백질의 발현 효율을 증진시키기 위한 융합 단백질에 관한 것이다. 보다 상세하게는, 목적단백질에 오량체형 독소단백질 및 hEPRS(Glutamyl-prolyl-tRNA synthetase from human)의 WHEP 도메인(EPRS 단백질의 중간 부분에 위치한 WHEP 도메인 TRS-1, TRS-2, TRS-3와 세 도메인을 연결하는 링커를 포함함)을 결합하여 제조된 융합단백질에 관한 것으로, 본 발명에 따른 hEPRS의 WHEP 도메인은 목적 단백질의 대장균에서의 발현을 위한 융합 파트너로 사용될 때, 목적 단백질의 수용성이 향상되었다.