Primer set for detecting PMMoV and method for detecting PMMoV using the same

The present invention relates to a primer set for an isothermal amplification reaction for detecting a pepper mild mottle virus (PMMoV), a composition, a kit, and a detecting method for detecting the PMMoV comprising the primer set. When the primer set according to the present invention is used to p...

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Bibliographische Detailangaben
Hauptverfasser:	BANG YOON HYUN, PETER PALUKAITIS, BAEK E SEUL
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a primer set for an isothermal amplification reaction for detecting a pepper mild mottle virus (PMMoV), a composition, a kit, and a detecting method for detecting the PMMoV comprising the primer set. When the primer set according to the present invention is used to perform the isothermal amplification method, the PMMoV can be specifically detected with high sensitivity than existing detecting methods, and a reaction time is greatly reduced to enable rapid and precise identification of the contamination and occurrence of the PMMoV. Accordingly, the present invention can be effectively utilized as an early detection system for peppers contaminated by the PMMoV. 본 발명은 고추 약한 모틀 바이러스(PMMoV)를 검출하기 위한 등온증폭반응용 프라이머 세트, 상기 프라이머 세트를 포함하는 고추 약한 모틀 바이러스(PMMoV) 검출용 조성물, 키트 및 검출방법에 관한 것이다. 본 발명에 따른 프라이머 세트를 이용하여 등온증폭법을 수행할 경우, 기존의 검출방법에 비하여 고추 약한 모틀 바이러스(PMMoV)를 높은 민감도로 특이적으로 검출할 수 있으며, 반응 시간이 크게 개선되어 고추 약한 모틀 바이러스(PMMoV)의 오염 및 발생 수준을 빠르고 정확하게 확인할 수 있다. 따라서, 본 발명은 고추 약한 모틀 바이러스(PMMoV)에 오염된 고추의 조기 검출 시스템으로서 효과적으로 활용될 수 있다.