Nile Tilapia Growth Performance Genotyping Kit

The present invention relates to a diagnostic kit for determining male and female growth characteristics of Oreochromis niloticus to provide high specificity. According to the present invention, the diagnostic kit comprises: an SNP Type 1: Mastermix 1 (DNA polymerase (0.5 U), 1× qPCR buffer, MgCl_2 (4 mM), dNTP Mix (dATP, dCTP, dGTP, dTTP; 600 mM each), and forward and reverse primers (0.5 μl each in sterile distilled water); an SNP Type 2: Mastermix 2 (DNA polymerase (0.5 U), 1× qPCR buffer, MgCl_2 (4 mM), dNTP Mix (dATP, dCTP, dGTP, dTTP; 600 mM each), and forward and reverse primers (0.5 μl each in sterile distilled water)); and an SNP Type 3: Mastermix 3 (DNA polymerase (0.5 U), 1× qPCR buffer, MgCl_2 (4mM), dNTP Mix (dATP, dCTP, dGTP, dTTP; 600 mM each), and forward and reverse primers (0.5 μl each in sterile distilled water). The Mastermixes include an SNP Probe 1 (0.5 μl, total, 120 μl), an SNP Probe 2 (0.5 μl, total, 120 μl), and an SNP Probe 3 (0.5 μl, total, 120 μl) as fluorescent probes, respectively, and includes ROX^TM dye (fluorescent dye) and sterilized distilled water. 본 발명은 나일 틸라피아의 암수 성장형질을 판별하는 진단키트에 있어서, 상기 진단키트는, SNP Type 1: Mastermix 1 [DNA 중합효소(0.5 U), 1× qPCR 완충액, MgCl2 (4 mM), dNTP Mix (dATP, dCTP, dGTP, dTTP; 각 600 mM), 순방향과 역방향 프라이머(각 0.5 μ멸균증류수]와, SNP Type 2: Mastermix 2 [DNA 중합효소(0.5 U), 1× qPCR 완충액, MgCl2 (4 mM), dNTP Mix (dATP, dCTP, dGTP, dTTP; 각 600 mM), 순방향과 역방향 프라이머(각 0.5 μ멸균증류수]와, SNP Type 3: Mastermix 3 [DNA 중합효소(0.5 U), 1× qPCR 완충액, MgCl2 (4 mM), dNTP Mix (dATP, dCTP, dGTP, dTTP; 각 600 mM), 순방향과 역방향 프라이머(각 0.5 μ멸균증류수]; 로 구성되고, 그리고 상기 각 Mastermix에 형광탐침자로서 각각 SNP Probe 1 (0.5 μ총 120 μl) , SNP Probe 2 (0.5 μ총 120 μl) , SNP Probe 3 (0.5 μ총 120 μl)를 포함하고, 또한 ROX™ dye (형광염료) 와 멸균증류수를 포함하여 이루어지는 나일 틸라피아의 암수 성장형질을 판별하는 진단키트에 관한 발명이다.