Novel IQ Gene Switch Without Gene Silencing

The present invention provides a system for inducing gene expression which is a system for regulating expression of a target gene in a host cell. The host cell comprises: an ecdysone receptor complex driver including (i) a DNA-binding domain, (ii) a transcriptional activation domain, and (iii) an ec...

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Bibliographische Detailangaben
Hauptverfasser:	HONG JEONG KWAN, HUR GANG MIN, SOHN KYUNG CHEOL, LEE JEONG SOO, RO HYUN JU, LEE JAE GEUN
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides a system for inducing gene expression which is a system for regulating expression of a target gene in a host cell. The host cell comprises: an ecdysone receptor complex driver including (i) a DNA-binding domain, (ii) a transcriptional activation domain, and (iii) an ecdysone receptor ligand binding domain; and an effector including (i) a response element capable of binding to the DNA-binding domain; and (ii) a target gene. Accordingly, the system for inducing gene expression which overcomes a problem of a Gal4-UAS-based system and is free from a gene silencing problem and cytotoxicity is provided to contribute to research on treatment mechanism and a treatment for incurable diseases. 본 발명은 본 발명은 숙주 세포에서 표적 유전자의 발현을 조절하는 시스템으로서, 상기 숙주세포는 (i) DNA-결합 도메인; (ii) 전사활성 도메인; 및 (iii) 엑디손 수용체 리간드 결합 도메인; 을 포함하는 엑디손 수용체 복합체 드라이버, (i) 상기 DNA 결합 도메인에 결합할 수 있는 반응 요소; 및 (iii) 표적 유전자; 를 포함하는 이펙터로 이루어지는 것을 특징으로 하는 유전자 발현 유도 시스템을 제공함으로써 Gal4-UAS 기반 시스템의 문제점을 극복하여 유전자 침묵 문제와 세포독성으로부터 자유로운 유전자 발현 유도 시스템을 제공함으로써 난치성 질환의 치료기전 및 치료제에 대한 연구에 기여할 수 있다.