Reverse transcriptase polymerase chain reaction for the detection of Middle East respiratory syndrome-related coronavirus and a diagnosing kit for Middle East respiratory syndrome-related coronavirus using the same

The present invention relates to: a reverse transcription (RT)-polymerase chain reaction (PCR) primer set for detecting the Middle East respiratory syndrome-related coronavirus (MERS-CoV) capable of diagnosing the MERS-CoV; a positive control gene for a false positive test; a MERS-CoV detection kit...

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Hauptverfasser: HAN SEO YOON, PARK JUNG HUN, YOO HYUN JOO, CHEON YOU JUNG, OH SO HEE, JEON YUN SEON, AN UK JIN, KIM JUNG HEE, PARK BYUNG IL, JUNG SUN DO, LEE SUN JIN, LEE SU BIN, OH JOO YEON, LEE SI WON, KIM JU HYUN, KIM SOON JI, LIM SU MIN, CHOI EUN SOL, PARK SOO HYUN, SEO JANG HOON, JANG HO YEONG
Format: Patent
Sprache:eng ; kor
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Zusammenfassung:The present invention relates to: a reverse transcription (RT)-polymerase chain reaction (PCR) primer set for detecting the Middle East respiratory syndrome-related coronavirus (MERS-CoV) capable of diagnosing the MERS-CoV; a positive control gene for a false positive test; a MERS-CoV detection kit including the same; and a detection method thereof. The primer set includes: a primer pair represented by SEQ ID NO: 1 and 7; a primer pair represented by SEQ ID NO: 10 and 12; and a primer pair represented by SEQ ID NO: 15 and 20. 본 발명은 중동호흡기증후군 코로나바이러스(Middle East respiratory syndrome-related coronavirus; MERS-CoV)를 진단할 수 있는 MERS-CoV 검출용 역전사(reverse transcription; RT)-중합효소연쇄반응(polymerase chain reaction; PCR) 프라이머 세트, 위양성 검정용 양성대조군유전자, 이를 포함하는 MERS-CoV 검출키트 및 검출방법에 관한 것이다.