RAPID DETECTION METHOD OF ANTI-DRUG ANTIBODY

The objective of the present invention is to provide a rapid detection kit to quantify an anti-drug antibody present in a sample in an accurate, simple, and rapid manner. The present invention comprises the steps of: (a) dissociating a complex of an anti-drug antibody and a drug by contacting a biol...

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Bibliographische Detailangaben
Hauptverfasser:	CHOI EUI YUL, LEE YOON SUK, LEE MI SOOK, SAYYED DANISHMALIK RAFIQ, CHON HYANG AH
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
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Beschreibung
Zusammenfassung:	The objective of the present invention is to provide a rapid detection kit to quantify an anti-drug antibody present in a sample in an accurate, simple, and rapid manner. The present invention comprises the steps of: (a) dissociating a complex of an anti-drug antibody and a drug by contacting a biological sample with an acid, the sample containing the complex of the anti-drug antibody and the drug; (b) forming a labeled drug-antibody-drug immune complex by contacting the complex-dissociated sample with a dry-form composition comprising a labeled drug and a drug to which a linker is bound; and (c) detecting a signal generated from the labeled immune complex. 본 발명은 정확하고, 간단하고, 신속한 방식으로 시료 내 존재하는 항-약물 항체를 정량화 할 수 있는 신속 검출 키트를 제공하는 것을 목적으로 한다. 본 발명은, (a) 항 약물 항체와 약물의 복합체를 포함하는 생물학적 시료를 산과 접촉시켜 상기 항 약물 항체와 약물의 복합체를 해리시키는 단계; (b) 상기 복합체가 해리된 시료를, 표지된 약물 및 링커가 결합된 약물을 포함하는 건조 형태의 조성물과 접촉시켜 약물-항체-약물의 표지된 면역 복합체를 형성하는 단계; 및 (c) 상기 표지된 면역 복합체에서 발생하는 신호를 검출하는 단계를 포함하는, 항 약물 항체(anti-drug antibody)의 신속 측정 방법을 제공한다.