DNA DNA polymerase variants that show high discrimination against genetic variation

The present invention relates to a DNA polymerase variant that belongs to family A and a use thereof. More specifically, the present invention relates to a DNA polymerase variant which easily undergoes polymerization when the base at the 3' end of a primer is complementary to a template, and ye...

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Bibliographische Detailangaben
Hauptverfasser:	KIM JAE JONG, CHA SUN HO, LIM SI KYU, LEE BO MI, KIM SEULKI, RYU JEOUNGHYUN
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a DNA polymerase variant that belongs to family A and a use thereof. More specifically, the present invention relates to a DNA polymerase variant which easily undergoes polymerization when the base at the 3' end of a primer is complementary to a template, and yet inhibits polymerization when the base at the 3' end of the primer is non-complementary to the template, thereby facilitating the discrimination between the two cases, a PCR process using the same, and a PCR kit comprising the same. The present invention is useful for genotyping of single nucleotide polymorphism (SNP), somatic mutation detection, and the like. 본 발명은 패밀리 A에 속하는 DNA 중합효소 변이체 및 그 이용에 관한 것으로서, 프라이머 3' 말단의 염기가 주형과 상보적일 때는 중합이 원활하게 일어나며, 비상보적일 때는 중합이 억제되어 두 경우의 구별 (discrimination)이 용이하게 되는 DNA 중합효소 변이체, 이 변이체를 이용한 PCR 방법 및 이 변이체를 포함하는 PCR 킷트에 관한 것이다. 본 발명은 단일염기다형성 분석 (SNP genotyping) 및 체세포 돌연변이 검출 (somatic mutation detection) 등에 유용하다.