6 Development of synthetic wheat generation protocol and its discriminant molecular marker

A method of breeding a hexaploid synthetic wheat by crossing a tetraploid durum wheat and a diploid goatgrass according to various embodiments of the present invention comprises the following steps: cutting and fixing spikes for emasculation; pollinating Ae.tauschii wheat with three or more spikes;...

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Bibliographische Detailangaben
Hauptverfasser:	TRUONG HAI AN, LEE WON JE, LEE HO JOUNG
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method of breeding a hexaploid synthetic wheat by crossing a tetraploid durum wheat and a diploid goatgrass according to various embodiments of the present invention comprises the following steps: cutting and fixing spikes for emasculation; pollinating Ae.tauschii wheat with three or more spikes; removing grains from the pollinated flowers and washing seeds; extracting immature embryos from the partial grains; transferring the embryo to a root medium; germinating the embryo; and transferring the germinated plant to a pot and treating with colchicine. 본 발명의 다양한 실시예에 따른 4배체인 듀럼밀과 2배체인 염소풀을 교배하여 6배체 합성밀을 육종하는 방법은, 스파이크를 잘라내고 고정하여 제웅(EMASCULATION)하는 단계; 3개 이상의 스파이크를 가진 Ae.tauschii 밀을 수분을 수분하는 단계; 수분 시킨 꽃에서 곡물을 꺼내고 종자를 세척하는 단계; 상기 일부 곡물에서 미성숙한 배아를 추출하는 단계; 상기 배아를 뿌리 배지에 옮기는 단계; 상기 배아를 발아시키는 단계; 및 발아된 식물을 화분에 옮기고 콜히친(COLCHICINE) 처리하는 단계;를 포함할 수 있다.