RECOMBINANT YEAST STRAIN HAVING STEROL PRODUCING ABILITY PREPARATION METHOD AND USES THEREOF
The present invention relates to a recombinant yeast strain having sterol productivity, a preparation method therefor and a use thereof and, more specifically, to a recombinant yeast strain capable of producing cholesterol and cholesterol precursors at a high yield through the deletion of ERG5 and E...
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| creator | MOON HYE YUN JANG HUI JEONG KANG HYUN AH JUNG SEON A |
| description | The present invention relates to a recombinant yeast strain having sterol productivity, a preparation method therefor and a use thereof and, more specifically, to a recombinant yeast strain capable of producing cholesterol and cholesterol precursors at a high yield through the deletion of ERG5 and ERG6 genes and the introduction of DHCR24 and DHCR7 genes by codon-optimizing same in multiple or with a codon context method, and a preparation method therefor and a use thereof. In addition, disclosed are a method for preparing a recombinant yeast strain with increased production yields of cholesterol and cholesterol precursors by the additional introduction of gene tHMG1, ERG2, ERG3, ERG27, or UPC2-1 in the prepared recombinant yeast strain, and a use thereof.
본 발명은 스테롤 생산능을 갖는 재조합 효모 균주, 이의 제조방법 및 용도에 관한 것으로, 더욱 상세하게는 ERG5 및 ERG6 유전자를 결손시키고 DHCR24 및 DHCR7 유전자를 다중으로 또는 코돈 콘텍스트 방식으로 코돈 최적화하여 도입함으로써 콜레스테롤 및 콜레스테롤 전구체를 높은 수율로 생산할 수 재조합 효모 균주와 이의 제조방법 및 용도에 관한 것이다. 또한 상기 제작된 재조합 효모균주에 tHMG1, ERG2, ERG3, ERG27, 또는 UPC2-1 유전자를 추가로 도입시킴으로 콜레스테롤 및 콜레스테롤 전구체 생산 수율이 증가된 재조합 효모 균주 제조 방법 및 용도에 관한 것을 포함한다. |
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본 발명은 스테롤 생산능을 갖는 재조합 효모 균주, 이의 제조방법 및 용도에 관한 것으로, 더욱 상세하게는 ERG5 및 ERG6 유전자를 결손시키고 DHCR24 및 DHCR7 유전자를 다중으로 또는 코돈 콘텍스트 방식으로 코돈 최적화하여 도입함으로써 콜레스테롤 및 콜레스테롤 전구체를 높은 수율로 생산할 수 재조합 효모 균주와 이의 제조방법 및 용도에 관한 것이다. 또한 상기 제작된 재조합 효모균주에 tHMG1, ERG2, ERG3, ERG27, 또는 UPC2-1 유전자를 추가로 도입시킴으로 콜레스테롤 및 콜레스테롤 전구체 생산 수율이 증가된 재조합 효모 균주 제조 방법 및 용도에 관한 것을 포함한다.</description><language>eng ; kor</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210209&DB=EPODOC&CC=KR&NR=20210014710A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210209&DB=EPODOC&CC=KR&NR=20210014710A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>MOON HYE YUN</creatorcontrib><creatorcontrib>JANG HUI JEONG</creatorcontrib><creatorcontrib>KANG HYUN AH</creatorcontrib><creatorcontrib>JUNG SEON A</creatorcontrib><title>RECOMBINANT YEAST STRAIN HAVING STEROL PRODUCING ABILITY PREPARATION METHOD AND USES THEREOF</title><description>The present invention relates to a recombinant yeast strain having sterol productivity, a preparation method therefor and a use thereof and, more specifically, to a recombinant yeast strain capable of producing cholesterol and cholesterol precursors at a high yield through the deletion of ERG5 and ERG6 genes and the introduction of DHCR24 and DHCR7 genes by codon-optimizing same in multiple or with a codon context method, and a preparation method therefor and a use thereof. In addition, disclosed are a method for preparing a recombinant yeast strain with increased production yields of cholesterol and cholesterol precursors by the additional introduction of gene tHMG1, ERG2, ERG3, ERG27, or UPC2-1 in the prepared recombinant yeast strain, and a use thereof.
본 발명은 스테롤 생산능을 갖는 재조합 효모 균주, 이의 제조방법 및 용도에 관한 것으로, 더욱 상세하게는 ERG5 및 ERG6 유전자를 결손시키고 DHCR24 및 DHCR7 유전자를 다중으로 또는 코돈 콘텍스트 방식으로 코돈 최적화하여 도입함으로써 콜레스테롤 및 콜레스테롤 전구체를 높은 수율로 생산할 수 재조합 효모 균주와 이의 제조방법 및 용도에 관한 것이다. 또한 상기 제작된 재조합 효모균주에 tHMG1, ERG2, ERG3, ERG27, 또는 UPC2-1 유전자를 추가로 도입시킴으로 콜레스테롤 및 콜레스테롤 전구체 생산 수율이 증가된 재조합 효모 균주 제조 방법 및 용도에 관한 것을 포함한다.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNisEKgkAQQL10iOofBjoHqwWdR3dsl3RXZsdACERiO0UJ9v9k0Ad0erzHWyZXpsLXuXXoBDrCIBCE0ToweLHuNBuxr6Bhr9viGzC3lZVuLtQgo1jvoCYxXgM6DW2gAGKIyZfrZHEfHlPc_LhKtiVJYXZxfPVxGodbfMZ3f-ZMZalS6eGYKtz_d30ArAUzYQ</recordid><startdate>20210209</startdate><enddate>20210209</enddate><creator>MOON HYE YUN</creator><creator>JANG HUI JEONG</creator><creator>KANG HYUN AH</creator><creator>JUNG SEON A</creator><scope>EVB</scope></search><sort><creationdate>20210209</creationdate><title>RECOMBINANT YEAST STRAIN HAVING STEROL PRODUCING ABILITY PREPARATION METHOD AND USES THEREOF</title><author>MOON HYE YUN ; JANG HUI JEONG ; KANG HYUN AH ; JUNG SEON A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_KR20210014710A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; kor</language><creationdate>2021</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>MOON HYE YUN</creatorcontrib><creatorcontrib>JANG HUI JEONG</creatorcontrib><creatorcontrib>KANG HYUN AH</creatorcontrib><creatorcontrib>JUNG SEON A</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>MOON HYE YUN</au><au>JANG HUI JEONG</au><au>KANG HYUN AH</au><au>JUNG SEON A</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>RECOMBINANT YEAST STRAIN HAVING STEROL PRODUCING ABILITY PREPARATION METHOD AND USES THEREOF</title><date>2021-02-09</date><risdate>2021</risdate><abstract>The present invention relates to a recombinant yeast strain having sterol productivity, a preparation method therefor and a use thereof and, more specifically, to a recombinant yeast strain capable of producing cholesterol and cholesterol precursors at a high yield through the deletion of ERG5 and ERG6 genes and the introduction of DHCR24 and DHCR7 genes by codon-optimizing same in multiple or with a codon context method, and a preparation method therefor and a use thereof. In addition, disclosed are a method for preparing a recombinant yeast strain with increased production yields of cholesterol and cholesterol precursors by the additional introduction of gene tHMG1, ERG2, ERG3, ERG27, or UPC2-1 in the prepared recombinant yeast strain, and a use thereof.
본 발명은 스테롤 생산능을 갖는 재조합 효모 균주, 이의 제조방법 및 용도에 관한 것으로, 더욱 상세하게는 ERG5 및 ERG6 유전자를 결손시키고 DHCR24 및 DHCR7 유전자를 다중으로 또는 코돈 콘텍스트 방식으로 코돈 최적화하여 도입함으로써 콜레스테롤 및 콜레스테롤 전구체를 높은 수율로 생산할 수 재조합 효모 균주와 이의 제조방법 및 용도에 관한 것이다. 또한 상기 제작된 재조합 효모균주에 tHMG1, ERG2, ERG3, ERG27, 또는 UPC2-1 유전자를 추가로 도입시킴으로 콜레스테롤 및 콜레스테롤 전구체 생산 수율이 증가된 재조합 효모 균주 제조 방법 및 용도에 관한 것을 포함한다.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | RECOMBINANT YEAST STRAIN HAVING STEROL PRODUCING ABILITY PREPARATION METHOD AND USES THEREOF |
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