RECOMBINANT YEAST STRAIN HAVING STEROL PRODUCING ABILITY PREPARATION METHOD AND USES THEREOF

The present invention relates to a recombinant yeast strain having sterol productivity, a preparation method therefor and a use thereof and, more specifically, to a recombinant yeast strain capable of producing cholesterol and cholesterol precursors at a high yield through the deletion of ERG5 and E...

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Bibliographische Detailangaben
Hauptverfasser:	MOON HYE YUN, JANG HUI JEONG, KANG HYUN AH, JUNG SEON A
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a recombinant yeast strain having sterol productivity, a preparation method therefor and a use thereof and, more specifically, to a recombinant yeast strain capable of producing cholesterol and cholesterol precursors at a high yield through the deletion of ERG5 and ERG6 genes and the introduction of DHCR24 and DHCR7 genes by codon-optimizing same in multiple or with a codon context method, and a preparation method therefor and a use thereof. In addition, disclosed are a method for preparing a recombinant yeast strain with increased production yields of cholesterol and cholesterol precursors by the additional introduction of gene tHMG1, ERG2, ERG3, ERG27, or UPC2-1 in the prepared recombinant yeast strain, and a use thereof. 본 발명은 스테롤 생산능을 갖는 재조합 효모 균주, 이의 제조방법 및 용도에 관한 것으로, 더욱 상세하게는 ERG5 및 ERG6 유전자를 결손시키고 DHCR24 및 DHCR7 유전자를 다중으로 또는 코돈 콘텍스트 방식으로 코돈 최적화하여 도입함으로써 콜레스테롤 및 콜레스테롤 전구체를 높은 수율로 생산할 수 재조합 효모 균주와 이의 제조방법 및 용도에 관한 것이다. 또한 상기 제작된 재조합 효모균주에 tHMG1, ERG2, ERG3, ERG27, 또는 UPC2-1 유전자를 추가로 도입시킴으로 콜레스테롤 및 콜레스테롤 전구체 생산 수율이 증가된 재조합 효모 균주 제조 방법 및 용도에 관한 것을 포함한다.