METHOD FOR IN VITRO DIAGNOSTIC DEVICE BY MEASURING FLUORESCENCE LIFETIME AND IN VITRO DIAGNOSTIC DEVICE FOR PERFORMING THE SAME

In an in vitro diagnosis method using a fluorescence time constant and an in vitro diagnosis system for the same, the in vitro diagnosis system comprises: a light source part for irradiating light onto a sample; a marking part for marking a biomarker on the fluorescence material expressed in the sample; an optical detecting part wherein the fluorescence material performs a fluorescence resonance energy transfer (FRET) reaction and converts the generated fluorescence signal into an electrical signal; a data acquisition part for collecting the electrical signal; a processing part for measuring the fluorescence time constant of the fluorescence material changed by the fluorescence resonance energy transfer reaction; and an extracting part for quantifying a concentration of the biomarker from the measured fluorescence time constant. Therefore, the present invention is capable of detecting more stable and accurate in vitro diagnosis information. 형광시정수를 이용한 체외 진단방법 및 이를 위한 체외 진단시스템에서, 상기 체외 진단시스템은 시료에 광을 조사하는 광원부, 상기 시료에서 발현된 형광물질에 바이오마커를 마킹하는 마킹부, 상기 형광물질들이 형광공명-에너지전이(fluorescence resonance energy transfer, FRET) 반응을 수행하여 생성된 형광신호를 전기신호로 변환하는 광 검출부, 상기 전기신호를 수집하는 데이터 획득부, 상기 형광공명-에너지전이 반응에 의해 변화되는 형광물질의 형광시정수를 측정하는 처리부 및 상기 측정된 형광시정수로부터 바이오마커의 농도를 정량화하는 추출부를 포함한다.