CRM197 CRM197 RECOVERY AND PURIFICATION METHOD OF BIOACTIVE CRM197 PROTEINS FROM INCLUSION BODIES INCLUDING CRM197

CRM197 CRM197 RECOVERY AND PURIFICATION METHOD OF BIOACTIVE CRM197 PROTEINS FROM INCLUSION BODIES INCLUDING CRM197

The present invention relates to a method for expressing and purifying insoluble proteins of CRM197. By solubilizing a CRM197 protein inclusion body using a non-modified solubilizing agent, it is possible to produce an active CRM197 protein with high yield. The mild solubilization method uses Tris-C...

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Bibliographische Detailangaben
Hauptverfasser: KOO BONG SEONG, SEO HYOUNG JONG, PARK AH REUM, JANG SEUNG WON, KIM JIN SOOK, PARK YOUNG GYUN, LEE HYEON CHEOL
Format: Patent
Sprache:eng ; kor
Schlagworte:
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Beschreibung
Zusammenfassung:The present invention relates to a method for expressing and purifying insoluble proteins of CRM197. By solubilizing a CRM197 protein inclusion body using a non-modified solubilizing agent, it is possible to produce an active CRM197 protein with high yield. The mild solubilization method uses Tris-Cl buffer, DMSO, n-propanol or sarkosyl solution. 본 발명은 CRM197의 불용성 단백질 발현 및 정제 방법에 관한 것이다. 본 발명은 비변성 가용화제를 이용하여 CRM197 단백질 봉입체를 가용화함으로써, 높은 수율로 활성형인 CRM197 단백질을 생산할 수 있다.