RNA MICRO-RNA ANALYSIS METHOD USING LIQUID CHROMATOGRAPHY

The present invention relates to a method for analyzing miRNA using liquid chromatography, which comprises the following steps: (A) preparing a sample solution containing miRNA; (B) pouring the sample solution into a column of liquid chromatography having a non-polar column as a stationary phase; an...

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Bibliographische Detailangaben
Hauptverfasser:	KIM, YOUNG SOO, KIM, DAE HYUN, YI, SANG WON
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a method for analyzing miRNA using liquid chromatography, which comprises the following steps: (A) preparing a sample solution containing miRNA; (B) pouring the sample solution into a column of liquid chromatography having a non-polar column as a stationary phase; and (C) supplying an organic solution and an aqueous solution as a mobile phase. The method of the present invention is capable of rapidly separating miRNA components, and is highly useful for analyzing samples containing a large number of miRNA components because of high separation efficiency. 본 발명은 액체 크로마토그래피를 이용한 miRNA를 분석하는 방법으로서, (A) miRNA를 포함하는 시료 용액을 준비하는 단계; (B) 고정상으로서 비극성 컬럼을 갖는 액체 크로마토그래피의 컬럼에 상기 시료 용액을 주입하는 단계; 및 (C) 이동상으로서 유기용액 및 수용액을 흘려주는 단계를 거쳐 miRNA 성분들을 신속하게 분리할 수 있으며, 분리도가 높아 다수의 miRNA 성분을 포함하는 시료 분석에 유용하다.