KIT FOR SPECIMEN-REAGENT RESPONSE ANALYSIS TARGET NUCLEIC ACIDS DETECTION APPARATUS AND METHOD USING THE SAME

The present invention relates to a kit for analyzing a sample-reagent reaction, which is convenient for performing a pretreatment process for a sample if necessary in a state where a sample and a reagent are contained in independent wells, respectively, and which is capable of stably performing a pretreatment process by preventing backflow, and easily analyzing a reaction after putting a reagent into a sample, and to an apparatus and a method for detecting target nucleic acid, which are configured to accurately detect target nucleic acid after performing a stable PCR amplification on the target nucleic acid by using the kit. To this end, the kit of the present invention comprises: a flow chip (110) having a first well (111) for containing a sample, a second well (112) for containing a reagent, and a flow path (113) for allowing the reagent of the second wall (112), which is overflowed, to be put into the first well (111); and a cover (120) for covering and sealing the flow chip (110) to prevent the reagent and the sample from being leaked, and having a filling member (122) formed on a portion covering the second well (112) to be filled in the second well (112) by push and allowing the reagent to be put into the first well (111), and a light transmitting window (121) formed on a portion covering the first well (111). A study related to the present invention is carried out by support of a core material source technology development project of Ministry of Trade, Industry and Energy (PNA-nanohybrid fusion material and molecular diagnosis system development for diagnosing femtomole level cancer genes, a project number: 10047831). Also, for the method for detecting target nucleic acid according to the present invention, a verification experiment applied to an actual clinical sample is carried out by receiving a clinical sample from a human-derived material resource bank of Incheon St. Mary′s Hospital. 본 발명은 시료 및 시약을 각각 독립적인 웰에 담은 상태로 필요시 시료에 대한 전처리 과정을 수행하기에 간편하고, 역류 방지에 의해서 전처리 과정을 안정적으로 수행할 수 있으며, 시약을 시료에 투입한 후 반응을 분석하기에도 용이한 시료 시약 반응 분석용 키트와, 이를 이용하여 표적 핵산을 안정적으로 PCR 증폭한 후 정확하게 검출할 수 있게 한 표적 핵산 검출 장치 및 방법에 관한 것으로서, 이를 위한 키트는 시료를 담아둘 제1 웰(111), 시약을 담아둘 제2 웰(112), 및 제2 웰(112)의 시약이 넘쳐 제1 웰(111)로 투입되게 할 유로(113)를 조성한 유동 칩(110); 시약 및 시료가 새지 아니하도록 유동 칩(11)을 덮어 실링하며, 제2 웰(112)을 덮는 부위에 조성되어 눌림에 의해 제2 웰(112)에 담겨 시약을 제1 웰(111)로 투입되게 할 담금 부재(122) 및 제1 웰(111)을 덮는 부위에 조성한 투광창(121)을 구비한 커버(120);를 포함하여 구성된다. 본 발명과 관련된 연구는 산업통상자원부 핵심소재원천기술개발사업(펨토몰 수준의 암유전자 진단을 위한 PNA-나노하이브리드