DNA Diagnostic method for marbling score by using DNA marker of keratin-associated protein 10-6 gene in Korean cattle

The present invention relates to a method of diagnosing a degree of marbling in Korean cows by using a DNA marker of a keratin-associated protein gene. By discovering a DNA molecular marker related to a degree of marbling for making an early prediction and diagnosis of genetic attributes in Korean c...

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Bibliographische Detailangaben
Hauptverfasser:	CHUNG, EUI RYONG, KIM, JUN SUNG, SHIN, SUNG CHUL
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a method of diagnosing a degree of marbling in Korean cows by using a DNA marker of a keratin-associated protein gene. By discovering a DNA molecular marker related to a degree of marbling for making an early prediction and diagnosis of genetic attributes in Korean cows with superior degree of marbling, the present invention provides a genetic testing method which can be used to improve accuracy in early selection of high-quality Korean cows and assessment of the individual animal by using the DNA marker. Specifically, the present invention provides a diagnosis method which detects SNP markers according to SNP genotypes of individual Korean cows via PCR-RFLP by using a specific SNP present in the keratin-associated protein 10-6 gene of Korean cow LOC515676, and determines individuals having the AA genotype as individuals having genetically superior degree of marbling compared to other individuals having AG and CC genotypes. 본 발명은 케라틴 연관 단백질 유전자 DNA 마커를 이용한 한우 상강도 진단 방법에 관한 것으로서, 상강도가 우수한 한우의 유전적 자질을 조기에 예측하고 진단하는 상강도 관련 DNA 분자표지를 발굴하여 이를 이용한 고품질 한우의 조기선발 및 개체 평가의 정확성을 제고하는 데 활용 가능한 유전자 검사 방법을 제공하기 위한 것이다. 더욱 상세하게는, 본 발명은 한우 LOC515676 (keratin-associated protein 10-6) 유전자 내에 존재하는 특정 SNP를 이용하여 PCR-RFLP 기법으로 한우 개체별 SNP 유전자형에 따른 단일염기다형 마커(SNP marker)를 검출하고, 이들 가운데 AA 유전자형을 가진 개체들을 AG 및 CC 유전자형을 가진 개체들에 비해 유전적으로 상강도 수치가 우수한 개체로 판정하는 진단 방법을 제공한다.