Method for Detection of Food Poisoning Bacteria By Using Gene Amplification and Kit for Use in The Same Method

The present invention relates to a method for detecting food poisoning bacteria using a gene amplification method and a kit used in the same method. According to the method of the present invention, nucleic acid is extracted from a sample and the extracted small amount of nucleic acid is molecule-co...

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Bibliographische Detailangaben
Hauptverfasser:	KO, YOUNG JUN, LEE, HUY HO, MYEONG, HYEON KOON
Format:	Patent
Sprache:	eng ; kor
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a method for detecting food poisoning bacteria using a gene amplification method and a kit used in the same method. According to the method of the present invention, nucleic acid is extracted from a sample and the extracted small amount of nucleic acid is molecule-concentrated through a whole genome amplification (WGA) reaction. So, the small amount of food poisoning bacteria, polluted by fish used as sliced raw fish, can be precisely, rapidly detected. 본 발명은 유전자 증폭 방법을 이용한 식중독 유발 세균의 검출 방법 및 이 방법에 사용되는 키트에 관한 것이다. 본 발명의 방법은 시료로부터 핵산을 추출하고, 추출된 미량의 핵산을 WGA (Whole Genome Amplification) 반응을 통해 분자농축을 함으로써, 횟감으로 사용되는 생선에 오염된 미량의 식중독 유발 세균을 정확하고 신속하게 검출할 수 있는 효과를 갖는다.